The increasing prevalence of peri-implantitis has led to a growing clinical use of implantoplasty, a procedure involving intraoral machining of the dental implant sur-face to remove biofilm. The absence of standardized clinical protocols may contribute to premature fatigue failure of dental implants. The present study aimed to determine the influence of machining depth on the cyclic mechanical behavior of dental im-plants. A total of 250 commercially pure Grade 3 titanium dental implants were dis-tributed into four groups according to machining depth: untreated (original), 0.2 mm, 0.4 mm, and 0.6 mm wall reduction. The implant system featured an internal connec-tion with a thread height of 0.4 mm. Finite element analysis was performed for each machining depth to evaluate Von Mises stress distribution and to simulate fatigue be-havior. The numerical models were validated through experimental fatigue testing using a servohydraulic MTS Bionix testing machine under ISO 14801:2016 standard conditions. Fractographic analysis was conducted by scanning electron microscopy. The results revealed that maximum Von Mises stresses were concentrated at the junc-tion between the implant thread and the implant body. The fatigue limit of the un-treated implants was approximately 400 N. Implants subjected to 0.4 mm machining exhibited a fatigue limit of 350 N, whereas lower fatigue limits were observed for 0.2 mm (290 N) and 0.6 mm (180 N) reductions. These findings demonstrate the signifi-cant mechanical effect of thread removal. At higher applied loads, fracture occurred in the coronal region of the implant, whereas at lower loads failure shifted to the im-plant–abutment connection. Finite element predictions showed high agreement with experimental results. The findings highlight a clinically relevant criterion: implanto-plasty depth should not exceed the original thread height, as excessive wall reduction markedly compromises fatigue resistance and long-term mechanical reliability.