Humanity is facing an unprecedented socio-ecological and climate crisis resulting from human impact on the planet, which requires a profound transformation in how we inhabit and develop our territories. Regenerative development is emerging as a key approach to strengthening living systems and improving environmental health. In this context, UNESCO Biosphere Reserves are consolidating their role as strategic instruments that link biodiversity conservation with sustainable development through integrated and par-ticipatory land management models. Mexico stands out for its regional and global leadership in implementing these areas. Participatory governance, promoted by the MAB program, encourages the active involvement of local communities. This article analyses the application of a regenerative and participatory design methodology in a Biosphere Reserve, evaluating both the process and the tools used. Beyond the fulfilment of sus-tainability objectives, it examines the lessons learned, results and scope from a regen-erative perspective, providing critical reflections on its effectiveness as a strategy for the socio-ecological management of vulnerable territories.