Submitted:
04 December 2025
Posted:
05 December 2025
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Abstract
Translationally silent ribosomes have become an important focus in RNA biology. Lacking mRNAs, they represent a hibernating ribosome state. Their quantification remains challenging. This protocol outlines how to measure silent ribosome induction under diverse conditions by analyzing RNA from polysome-profiling fractions in yeast and mammalian cells, using RNA-seq with detection and removal of anomalously amplified rRNAs, complemented by qPCR for validation. For an example of the execution of this protocol, please refer to Rahaman, et. al [1].
Keywords:
monosome
; macrophage
; 5S rRNA
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