This study investigates the differences in flexural behavior of ultra-high-performance concrete (UHPC) arising from variations in test methods and key experimental parameters. Flexural tensile tests were conducted on 51 specimens representing 17 combinations of test variables, including steel fiber length (13 mm and 19.5 mm), specimen cross-sectional dimensions (75×75 mm, 100×100 mm, and 150×150 mm), presence or absence of a notch, and loading configuration (three-point and four-point loading). The tests were performed in accordance with ASTM C1609 and EN 14651, and both deflection and crack mouth opening displacement (CMOD) were normalized by the span length to compare the influence of each parameter. The notched specimens demonstrated significantly improved reliability, exhibiting up to an 8.4-fold reduction in standard deviation due to the consistent initiation of cracking. Regarding size effects, the 75×75 mm specimens showed an overestimation of flexural performance due to the wall effect of fiber distribution, whereas the 100×100 mm and 150×150 mm specimens exhibited similar flexural responses. The comparison of loading configurations revealed that three-point loading produced up to 11.7% higher flexural tensile strength than four-point loading, attributable to concentrated moment–shear interaction and the combined effects of fiber bridging and shear resistance mechanisms. In addition, specimens with longer steel fibers (19.5 mm) exhibited 5.2–9.7% higher flexural performance than those with shorter fibers (13 mm), which is attributed to enhanced interfacial bonding and improved crack dispersion capacity.