3.1. Effect of Addition of Enzymes Versus Thermal Drying
The FTIR spectra (
Figure 1) shows clearly that the nature of the organic matter in the food waste has changed as result of the addition of the enzyme. Significant differences in the height of the various band numbers and the ratios of the heights and the assignment of the FTIR bands are given in
Table 2.
The results indicate that changes occurred mostly in the soluble and easily decomposable organic matter e.g., carbonyl 1740 cm-1, alkyl 2922 cm-1, with little or no change in the aromatic structure 1630 cm-1. Furthermore, the ratio of the peaks 1740cm-1/1071cm-1 decreased. This indicates an overall decrease in the carbonyl content upon treatment. Similarly, there was a decrease in the ration 2920cm-1/1620cm-1 showed the relative enrichment of aromatic structure vis a vis alkyl structure.
Table 1.
Reduction (or increase) of the height of selected wave numbers and ratios, as a result of the addition of enzyme.
Table 1.
Reduction (or increase) of the height of selected wave numbers and ratios, as a result of the addition of enzyme.
Wave Number cm-1
|
Significance Level |
3200 |
p=0.086 NS OH (phenols, carboxylic groups) |
2930 |
p=<0.001 C-H stretching of alkyl structures |
2830 |
p=<0.001 C-H stretching of alkyl structures |
|
C=O stretching of carboxyl, acids and ketones |
1720 |
p=<0.001 ketones |
1620 |
NS Aromatic C=C. COO-, C=O |
1460 |
p=<0.0001 C-H stretching of aiphatic structures |
1148 |
p=<0.001 C-O stretching of polysaccharides |
1017 |
p=<0.002 Increase silica, carbohydrates |
2920/1620 |
p=<0.001 |
1720/1017 |
p=0.001 |
These results are in agreement with the findings of Zhang et al. 2008, which showed inoculation albeit with microorganism accelerated the organic matter degradation. However, their results were over a longer period of time.
The effect of enzyme addition on extractible nutrients and electrical conductivity (see
Table 3) showed no statistically significant effect although there were trends towards slight increase, however pH was trending upwards.
Table 2.
Effect of enzyme addition on pH, EC and extractable N, P and K.
Table 2.
Effect of enzyme addition on pH, EC and extractable N, P and K.
|
pH |
EC |
Ext.N |
Ext.P |
Ext.K |
|
|
μS/cm |
mg/L |
mg/L |
mg/L |
Untreated |
5.0 |
7793 |
35.8 |
973 |
5566 |
treated |
5.3 |
8250 |
44.2 |
1360 |
7541 |
Sig. |
x |
NS |
NS |
NS |
NS |
The heavy metal content levels of the Harp product are extremely low, see
Table 3. In the context of the new EU fertilizer regulations [REGULATION EU 2019/1009),], we can see they are very well within any tolerance or required levels.
The analysis of plant nutrient content over a longer period of time (over 4 years) indicated reasonable levels of N, P and K and the contents fall within the criteria of the new fertilizer regulations NPK index of 4.1 [NPK Index EU Fertilizer Regulations 2019e],
Table 4.The carbon content of the sample is very high indicating little or no loss of carbon during the process.
Emissions
During the processing of food waste, emissions were minimal, with ammonia <1 ppm, H2S < 0.1 ppm, amines <0.5 ppm and mercaptans <0.5ppm. Much of the carbon is retained within the substrate in the form of organic carbon and therefore mitigating much of the carbon volatilization in the form of CO2 emissions. Total Dust and Respirable Dust was a % of TWA limit < 2.1 and 7.91 respectively. There was no change in Volatile organic Carbon after 8 hours reference was 0.278 mg/m3 (Report available as supplementary information) [Axis Environmental Services. Limerick 2016).]. As mentioned above, processing of the food waste, emissions were minimal with theoretical reductions in Methane and Nitrous Oxide of 46-69% and 73% compared to traditional composting methods respectively [Cerda et al., 2018.Cao, Y. et al., 2019 ,EEA Report No 3/2011,Hwang et al.,2020] Cerda et., al has shown that composting can lead to significant amounts of GHG emissions
Plant Growth Trial
The pH analysis for the Harp material, at 5.5 is low for what would typically be expected for a compost (
Table 5). The EC for the Harp material is very high potentially indicating high salt levels due to higher nutrient content. The organic matter content is very high compared to BWC and CGW 2 to 4 times higher than the BWC and CGW respectively.
Extractable Nitrogen content are much higher in the Harp product and lowest in CGW (
Table 6)
Table 6.
Available nitrogen content of materials tested.
Table 6.
Available nitrogen content of materials tested.
|
|
Ammonium (NH4+) |
Nitrate (NO3-) |
Material |
|
Dry Sample (mg/Kg) |
Fresh Sample (mg/L) |
Dry Sample (mg/Kg) |
Fresh Sample (mg/L) |
Harp product |
|
427.4 |
210 |
103.8 |
51 |
Greenwaste |
|
79 |
20 |
122.5 |
31 |
BioWaste |
|
227.5 |
128.5 |
211.5 |
119.5 |
Table 7.
Influence of application rates on volume basis on the addition of total N and available macronutrients and organic matter regarding the pot trial.
Table 7.
Influence of application rates on volume basis on the addition of total N and available macronutrients and organic matter regarding the pot trial.
|
Application Rate (Volume/ml) |
Weight of Addition (g) |
Dry Weight of Addition (g) |
TKN (Addition) (g) |
NH4 (Addition) (g) |
NO3 (Addition) (g) |
P (Addition) (g) |
K (Addition) (g) |
Organic Matter (g) |
Organic Carbon (g) |
Harp product (4%) |
80 |
45.6 |
39.31 |
1.217 |
0.0168 |
0.0041 |
0.0481 |
0.4720 |
35.179944 |
20.439744 |
Harp product (8%) |
160 |
91.2 |
78.61 |
2.435 |
0.0336 |
0.0082 |
0.0962 |
0.9440 |
70.359888 |
40.879488 |
Harp product (12%) |
320 |
136.8 |
117.92 |
3.652 |
0.0504 |
0.0122 |
0.1443 |
1.4160 |
105.539832 |
61.319232 |
Greenwaste (4%) |
80 |
46.16 |
20.22 |
0.472 |
0.0016 |
0.0025 |
0.0060 |
0.1707 |
9.1992264 |
5.3577912 |
Greenwaste (8%) |
160 |
92.32 |
40.44 |
0.944 |
0.0032 |
0.0050 |
0.0120 |
0.3414 |
18.3984528 |
10.7155824 |
Greenwaste (12%) |
320 |
138.48 |
60.65 |
1.416 |
0.0048 |
0.0074 |
0.0180 |
0.5120 |
27.5976792 |
16.0733736 |
Biowaste (4%) |
80 |
49.6 |
45.19 |
0.616 |
0.0103 |
0.0096 |
0.0034 |
0.1117 |
10.392688 |
6.0548704 |
BioWaste (8%) |
160 |
99.2 |
90.37 |
1.232 |
0.0206 |
0.0191 |
0.0067 |
0.2233 |
20.785376 |
12.1097408 |
Biowaste (12%) |
320 |
148.8 |
135.56 |
1.848 |
0.0308 |
0.0287 |
0.0101 |
0.3550 |
31.178064 |
18.1646112 |
The data over the 4 harvests (2 harvests for CGW) over 160 days showed that the Harp material has a consistently higher fresh weight than the comparative compost treatments indicating sustained availability of nutrients from the material,
Figure 2.
As expected, nitrogen uptake (
Figure 3) effectively mirrored fresh weight returns. As the experiment progressed through the harvests, the impact of application rate and initial nitrogen application seem to have had an impact. In terms of recovered nitrogen from the grass samples harvested from each pot, the comparative nitrogen uptake in the pots with Harp material applied to the biowaste pots was 12.5 times higher at 4% application rate, 7.44 times higher at 8% application rate, and 4.6 times higher at 12% application rate.
Therefore, the comparative efficacy in terms of nitrogen uptake is more pronounced at lower application rates. Given that there will possibly be agronomic and environmental constraints on applications at the tested higher rates. The performance at the lower rate of treated are significant. Uptake of P and K followed a similar pattern to N uptake.
With regards to phosphorus and potassium uptake, due to the application rates of P and K being variable between the three materials due to different P and K content despite the rates on volume basis being similar it followed a similar scenario to N uptake (
Figure 4). Significantly more available phosphorus was present in the Harp material than in the CBW or the CGW material. The recovery (uptake) of phosphorus and to a lesser extent potassium from materials subjected to the Harp treatment process may be beneficial from a purely agronomic and resource utilization perspective. Given the lack of naturally occurring phosphorus resources within the EU, greater emphasis is being placed on technologies and processes for the recovery and utilization of phosphorus from organic waste streams.
With regards to magnesium, calcium, and Sulphur uptake, similar to the scenario with nitrogen, it is an indication that the elements were available to be taken up by the plant. Data available as supplementary information.
Figure 5.
Post cropping soil content and characterisation analysis of Harp material and composts.
Figure 5.
Post cropping soil content and characterisation analysis of Harp material and composts.
In relation to the residual organic matter in the soil, the application of Harp materials had a positive impact on organic matter levels, which was expected given the high organic matter content on the material. Despite the higher macronutrients uptake by plants during the cropping period, the fact that there is still residual nitrogen (total N), and extractable phosphorus left after cropping comparable to BioWaste compost is of interest and reflective of the comparatively high nutrient value of the Harp material. (GW compost not comparable as only two harvests taken due to lack of growth and minimal nutrient uptake by grass occurred)
On average, food waste processed using this Harp technology achieve, a Total Organic Carbon (TOC) of over 45% (n=70). Comparing this to the Carbon content of a number of bio-waste and greenwaste compost from Ireland, UK and Switzerland of 23% to 34% (Prasad et al., 2012)e the levls of organic matter is very high.)With over 70 analyses performed on Harp treated food waste, the median percentage N-P
2O
5-K
2O values are estimated at 2.8-0.8-1.5 or an NPK index of 5.1% as shown in
Table 4 which meet the minimum nutrient limit of 4% in the EU Fertiliser Regulations 2019/1009 [] and the minimum nutrient value for all fertiliser regulations across the United States [SUMMARY STATE FERTILIZER LAWS 2003)].