Submitted:
07 September 2024
Posted:
12 September 2024
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Abstract
Objectives: the aim of this study was the assessment and monitoring of the safety and quality of the existing water supplies in Ibb and Taiz governorates from the microbial side and testing the quality of drinking water in the Ibb and Taiz governments and comparing it with the World Health Organization. Methods: the water samples (99 samples) taken from some water sources, distribution reservoirs, and different regions were studied for bacteriological analysis, in which they were inoculated into culture media using two methods: the presumptive test for the most probable number and the confirmatory test for bacterial identification. Results: the results indicated the non-existence of indicator bacteria that were used to indicate the possible presence of fecal bacteria among 24 (24.24%) water samples taken from Taiz governorate, while the presence of an unacceptable number of Escherichia coli in house tanks (30%) was indicated among 75 (74.25%) water samples taken from Ibb governorate. Conclusions: the results of the study in Taiz governorate show that the water is fit for human consumption, as well as the samples taken from water sources and distribution networks in Ibb governorate, but the results of the samples taken from house tanks showed that the water is not fit for human consumption and needs to be treated.
Keywords:
Escherichia coli
; Coliform
; Total Bacterial Count
; Presumptive Test
; Taiz
; Ibb
; Yemen
Introduction
Water is a natural resource that is critical to human survival (Adimall and Qian, 2022; Ugwu et al., 2017). It sustains all forms of life and generates jobs and wealth in the water, tourism, and recreation industries. Te global slogan “Water is Life” implies that water is one of the most basic human needs. Life as we know it on our planet would be impossible without water (Adimalla et al., 2022; Bekele et al., 2018).
Water is an essential natural resource for humans to live (Adimall, and Qian, 2022; Ugwu et al., 2017). Water nourishes all forms of life and contributes to the economic growth of several industries such as water, tourism, and recreation. The worldwide expression "Water is Life" demonstrates the idea that water is an essential and fundamental requirement for the continued existence of people. Water is essential for supporting life on our planet (Adimalla et al., 2022; Bekele et al., 2018).
The high clay content of the soil, which results in a slow rate of water percolation and a significant distance to the groundwater, will lead to a greater likelihood of bacterial survival compared to the well-aerated sand. Nevertheless, the extended duration of the flow permits ample time for the water to undergo filtration prior to its entry into the groundwater (Adetunde and Glover, 2010). A multitude of significant challenges that jeopardize the existence of humanity on Earth arise from the scarcity of potable water in numerous areas, along with the degradation of environmental aesthetics (Adimalla 2020; Bote and Desta, 2022).
Microorganisms have a significant impact on the quality of water, particularly in relation to waterborne diseases. The specific bacteria that are associated with these diseases include Salmonella spp., Shigella spp., Escherichia coli, and Vibrio cholera. These factors contribute to the occurrence of typhoid fever, diarrhea, dysentery, gastroenteritis, and cholera (Adetunde and Glover, 2010). Consequently, water is analyzed microbiologically in order to assess its hygienic condition and its appropriateness for common purposes (Ohanu et al., 2012). Yemen is classified as a developing country that does not have effective policies or programs in place to manage or prevent the spread of disease-causing microbes among its people (Al-Hadheq et al., 2023; Mengstie et al., 2020). The World Health Organization (WHO) reports that over 30% of the global population lacks access to potable water. Every year, 829,000 individuals suffer to diarrhea caused by a drink of contaminated drinking water, inadequate sanitation, and poor hand hygiene (WHO, 2019).
In order to provide a safe source of drinking water, it is essential to perform observation for the existence of pathogens. Nevertheless, doing a comprehensive examination of the water supply for each individual bacterium would be costly and time-consuming. Instead, an indicator organism is employed to signal the potential existence of harmful bacteria, which are capable of causing diseases (Charles et al., 2005). In this current research, we have performed microbiological analysis of drinking water samples from Taiz and Ibb governorates to assessment of the quality drinking water from bacterial side.
Materials and Methods
Study Area and Period
This cross-sectional study was carried out in different areas of water supply in the Taiz and Ibb governorates, Yemen, during the period from January to February 2023. In total, 99 water samples from different sources, distribution networks, and house tanks in Taiz and Ibb governorates were collected for bacterial analysis.
Sample Collection
Drinking water samples were aseptically collected in a sterilized 300-mL capacity and placed in an insulated cold box for transport to a water testing laboratory. Water samples are examined as soon as possible on arrival and always within 6 hours of collection (Lansing et al., 2005).
Microbiological Analysis
For the total bacterial count, about one mL of each water sample was separately inoculated onto a plate containing 25 mL of nutrient agar with 1 mL of water sample and incubated at 37°C for 72 hours. The total bacterial visible colonies were counted after the incubation period according to the following formula: CFU/mL = (Number of colonies × dilution factor) / volume of culture plate.
Furthermore, the detection of Enterococcus faecalis was performed by inculcating one mL of a water sample into 10 mL of Azide dextrose broth and incubating at 37°C for 24 hours. Investigation of Escherichia coli: One mL from the water sample was inculcated into 10 mL of MacConkey broth and incubated at 37°C as a presumptive test for 48 hr. In addition, the Eosin Methylene blue was inoculated by loop and incubated at 37°C for 24 h to check the positive presumptive test. The isolation of coliform was done by using MacConkey agar that differentiates between lactose and non-lactose fermenter organisms, where coliforms are lactose fermenters and produce pink colonies on this medium after the incubation period. The quality control of each process was done by incubating two control plates for checking the sterility of the samples.
Statistical Analysis
The data collected from the results of microbiology tests, were analyzed by using SPSS Version 16. P-values of less than 0.05 (P<0.05) were considered statistically significant.
Results
Results of Studied Samples from Taiz Government
The fecal bacteria were not present in all studied water sources, but the high total bacterial counts were in Al-Hoban, followed by Kalaba, and the low number was in Al-Dabab, as shown in Table 1. Our finding showed that the relationship between the free chlorine ratio and the total bacterial count was variable in some regions, where the high ratio of free chlorine was in the distribution reservoir and the low ratio was in Bab Mosa, whereas in Al-Shamasi, Al-Markazi, and Kalaba, the ratio was variable. However, the total bacterial count was high in Bab Mosa, while in Kalaba, Al-Shmasi, and the distribution reserve, it was the same. It also shows no growth of the fecal bacteria in all studied regions.
The total bacterial count before and after sterilization and the effectiveness of sterilization showed that the effectiveness of sterilization was high in Al-Markazi, low in Bab Mosa, and variable in other regions, as shown in Table 2.
Results of Studied Samples from Ibb Government
The number of bacteria in samples collected from different sources of water in the Ibb government was higher in Al-Sign Al-Markazi well and lower in Al-Salaba; however, there was no growth of fecal bacteria in all studied water samples, as shown in Table 3.
Our finding showed that the number of bacteria in samples taken from the distribution network of five regions supplied by Al-Salaba and Al-Mala'ab wells was higher in the Mophleh line, followed by Al-Madariah, Alodain Street, and Al-Wazeiah regions, but the lower number was in the Al-Mohafadah region. However, the fecal bacteria were not present in all regions, as shown in Table 4.
Our results showed that there where the higher number of total bacteria and E. coli were present in Al-Mohafadah region and the lower number were in Al-Wazeiah region, however, the Enterococcus fecalis was not present, as showing as in Figure 1.
The number of bacteria in samples taken from the distribution network of the two regions was supplied by Al-Signs Al-Markazi well, where a higher number was present in the Wadi Al-Dahab region and a lower number in the Al-Signs Al-Markazi region, and Enterococcus faecalis was not present, as shown in Table 5. Our findings showed that the number of bacteria in samples taken from the distribution network of two regions supplied by Al-Signs Al-Markazi well, where a higher number was present in the Wadi Al-Dahab region and a lower number in the Al-Signs Al-Markazi region and Enterococcus faecalis was not present.
These results showed that the higher number was present in the Akamat Easa region, followed by the Haratha region, while the lower number was in the Dar Al-Sharaf region. However, the fecal bacteria were not present in all studied regions, as shown in Table 6.
Finally, the number of bacteria in samples taken from houses in tanks of three regions was supplied by seven and eight wells, where the higher number of total bacteria was present in the Haratha region and the lower number was present in Akamat Easa; however, the number of Escherichia coli was high in Haratha, Akamat Easa, and Dar Al-Sharaf, as shown in Table 7.
Discussion
The occurrence of waterborne diseases in developed countries is generally low due to a generally good system of water treatment, distribution, and monitoring. Waterborne diseases are among the leading causes of morbidity and mortality in low- and middle-income countries, frequently called developing countries.
Developed countries typically have good systems for treating, distributing, and monitoring water, which helps to reduce the incidence of waterborne illnesses. Low- and middle-income nations, often known as developing countries, have one of the highest rates of morbidity and mortality from waterborne diseases.
In wealthy countries, the incidence of waterborne infections is often low due to an effective system of water treatment, distribution, and monitoring. Waterborne infections are a significant source of illness and death in poor and middle-income nations, sometimes referred to as developing countries (Adimall, and Qian, 2022). Yemen, such a developing country, suffers from a lot of water-related health problems. Contaminated or polluted water is water that contains poisonous chemicals, pathogenic organisms, industrial waste, or sewage. The majority of diseases in underdeveloped nations can be attributed to the absence of potable water, such as typhoid, cholera, and hepatitis (Ohanu et al., 2012; Penna et al., 2002).
In Taiz governorate, the results of the study showed no growth of indicator bacteria in all studied water samples. This means that these waters are free from pathogenic bacteria. Thus, these waters are drinkable and fit for human consumption from a bacteriological perspective. In addition, the total bacterial count was high in Bab Mosa, while in Kalaba, Al-Shmasi, and the distribution reserve, it was the same. It also shows no growth of the fecal bacteria in all studied regions. Similar findings were reported in Thailand (Mengstie et al., 2020) and Iran (Shahryari et al., 2020). Other studies have also reported the high bacteria count in water in Iran (Kouchesfahani et al., 2015; Moazeni et al., 2012).
In addition, the present study found that there was no growth of indicator bacteria in all samples taken from the Ibb Governorate wells and distribution network, but the samples taken from house tanks (10%) showed growth of E. coli, which indicates that the water is unacceptable for human consumption according to WHO (WHO, 2015). This could be attributed to the presence of gelatinous layers accumulating on the walls of the tank, resulting in turbidity (Lewandowski and Beyenal, 2007). Similar findings were reported in Iraq (Aldhamin, 2023) and Pakistan (Shafique et al., 2020). In addition, other reports revealed that most bacteria contaminating drinking water in Aden and Hadhramout governorates were E. coli (Hassan et al., 2008; Bin Hamed and Bubakr, 2019). A high rate of E. coli was reported in drinking water in Thailand (Yongyod et al., 2023) and Indonesia (Alfian et al., 2023).
Author Contributions
Al-Hadheq and Al-Abood Conceived and designed the experiments. Al-Hadheq performed the experiments: Al-Hadheq, Al-Aboud, and Al-Arabi analyzed the data and wrote the first draft of the manuscript. Al-Maktari contributed to the writing of the manuscript. Al-Hadheq, Al-Asbahi, and Edress agree with the manuscript results and conclusions. All authors have read, revised, and approved the final manuscript.
Data Availability
The original data source could be shared upon the request of the principal investigator.
Consent for Publication
Not applicable.
Competing Interests
The authors declare that they have no competing interests.
Ethics Approval and Consent to Participate
The study protocol was approved by the medical microbiology department, faculty of sciences, Ibb University.
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Figure 1.
The total bacterial count and E. coli of samples taken from different regions that supplies by Al-Salaba and Al-Mala'ab wells.
Figure 1.
The total bacterial count and E. coli of samples taken from different regions that supplies by Al-Salaba and Al-Mala'ab wells.
Table 1.
The bacterial count of samples taken from some water sources in Taiz government.
| Water Sources | TBC ×10² per 100 mL | Fecal Bacteria/100 mL | ||||
|---|---|---|---|---|---|---|
| Number of Samples | Mean | Coliform/ E. coli | E. Fecalis | |||
| 1st | 2nd | 3rd | ||||
| Kalaba | 16 | 10 | 11 | 12 | 0 | 0 |
| Al Dapab | 5 | 13 | 6 | 8 | 0 | 0 |
| Hoban | 15 | 18 | 20 | 18 | 0 | 0 |
TBC = Total Bacterial Count.
Table 2.
The total bacterial count before and after sterilization and the effectiveness of sterilization.
Table 2.
The total bacterial count before and after sterilization and the effectiveness of sterilization.
| Regions | TBC ×10² per 100 mL | Effectiveness of Sterilization | |
|---|---|---|---|
| Before sterilization | After sterilization | ||
| Distribution Reservoir | 12 | 2 | 83.3% |
| Bab Mosa | 12 | 4 | 66.7% |
| Al-Shamasi | 12 | 2 | 83.3% |
| Al-markazi | 12 | 1 | 91.7% |
| Kalaba | 12 | 2 | 83.3% |
TBC = Total Bacterial Count.
Table 3.
The number of bacteria of samples taken from some water sources in Ibb governorate.
| Wells | Total Bacteria ×10² per 100 mL | Fecal Bacteria/100 mL | ||||
|---|---|---|---|---|---|---|
| Number of Samples | Mean | Coliform/ E. coli | E. Fecalis | |||
| 1st | 2nd | 3rd | ||||
| Al-Salaba | 0 | 2 | 1 | 1 | 0 | 0 |
| Al-Mala'ab | 4 | 11 | 16 | 10 | 0 | 0 |
| Al-Segn Al-Markazi | 11 | 15 | 9 | 11 | 0 | 0 |
| Seven | 10 | 6 | 6 | 7 | 0 | 0 |
| Eight | 7 | 5 | 10 | 7 | 0 | 0 |
Table 4.
The bacterial count in samples taken from distribution network of different regions that supplies by Al-Salaba and Al-Mala'ab.
Table 4.
The bacterial count in samples taken from distribution network of different regions that supplies by Al-Salaba and Al-Mala'ab.
| Region | Total Bacteria ×10² per 100 mL | Fecal Bacteria/100 mL | ||||
|---|---|---|---|---|---|---|
| Number of Samples | Mean | Coliform/ E. coli | E. Fecalis | |||
| 1st | 2nd | 3rd | ||||
| Alodain street | 31 | 19 | 25 | 25 | 0 | 0 |
| Al-Madriah | 45 | 42 | 37 | 41 | 0 | 0 |
| Almohafadah | 16 | 13 | 21 | 16 | 0 | 0 |
| Mophleh line | 56 | 61 | 63 | 60 | 0 | 0 |
| Al-Wazeiah | 12 | 17 | 38 | 22 | 0 | 0 |
Table 5.
The bacterial count of samples taken from distribution network of different regions that supplies by Al-Segn Al-Markazi wells.
Table 5.
The bacterial count of samples taken from distribution network of different regions that supplies by Al-Segn Al-Markazi wells.
| Region | Total Bacteria ×10² per 100 mL | Fecal Bacteria/100 mL | ||||
|---|---|---|---|---|---|---|
| Number of Samples | Mean | Coliform/ E. coli | E. Fecalis | |||
| 1st | 2nd | 3rd | ||||
| Al-Segn Al-Markazi | 51 | 56 | 72 | 59 | 0 | 0 |
| Wadi Al-Dahab | 82 | 75 | 87 | 81 | 0 | 0 |
Table 6.
The bacterial count of samples taken from distribution network of different regions that supplies.
Table 6.
The bacterial count of samples taken from distribution network of different regions that supplies.
| Region | TBC ×10² per 100 mL | Fecal Bacteria/100 mL | ||||
|---|---|---|---|---|---|---|
| Number of Samples | Mean | Coliform/ E. coli | E. Fecalis | |||
| 1st | 2nd | 3rd | ||||
| Haratha | 55 | 58 | 64 | 59 | 0 | 0 |
| Dar Al-Sharaf | 42 | 38 | 54 | 44 | 0 | 0 |
| Akamt Easah | 63 | 54 | 71 | 62 | 0 | 0 |
Table 7.
The bacterial count in samples of houses tanks from different regions that supplies by Seven and Eight wells.
Table 7.
The bacterial count in samples of houses tanks from different regions that supplies by Seven and Eight wells.
| Region | Number of samples | Mean | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 1st | 2nd | 3rd | |||||||
| TBC ×102 |
E.coli | TBC ×102 |
E.coli | TBC ×102 |
E.coli | TBC ×102 |
Coliform/ E. coli | E. fecalis | |
| Haratha | 85 | 16 | 82 | 18 | 73 | 11 | 80 | 15 | 0 |
| Dar Al-Sharaf | 65 | 23 | 81 | 29 | 69 | 10 | 71 | 21 | 0 |
| Akamt Easah | 59 | 12 | 67 | 14 | 70 | 20 | 65 | 15 | 0 |
TBC = Total Bacterial Coun.
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