Version 1
: Received: 8 May 2024 / Approved: 8 May 2024 / Online: 10 May 2024 (03:41:23 CEST)
How to cite:
Park, Y.; Park, T.; Lee, S.; Yun, M.; Kim, B. Protective Effects of Allium senescens Extract against 6-Hydroxydopamine in Neurons. Preprints2024, 2024050558. https://doi.org/10.20944/preprints202405.0558.v1
Park, Y.; Park, T.; Lee, S.; Yun, M.; Kim, B. Protective Effects of Allium senescens Extract against 6-Hydroxydopamine in Neurons. Preprints 2024, 2024050558. https://doi.org/10.20944/preprints202405.0558.v1
Park, Y.; Park, T.; Lee, S.; Yun, M.; Kim, B. Protective Effects of Allium senescens Extract against 6-Hydroxydopamine in Neurons. Preprints2024, 2024050558. https://doi.org/10.20944/preprints202405.0558.v1
APA Style
Park, Y., Park, T., Lee, S., Yun, M., & Kim, B. (2024). Protective Effects of <em>Allium senescens</em> Extract against 6-Hydroxydopamine in Neurons. Preprints. https://doi.org/10.20944/preprints202405.0558.v1
Chicago/Turabian Style
Park, Y., Mihae Yun and Boyong Kim. 2024 "Protective Effects of <em>Allium senescens</em> Extract against 6-Hydroxydopamine in Neurons" Preprints. https://doi.org/10.20944/preprints202405.0558.v1
Abstract
Oxidative neurodegeneration causes various neuronal diseases including Alzheimer's disease, Parkinson's disease, and Huntington's disease. This study aimed to demonstrate the protective effect of leaf extracts of Allium senescens on 6-hydroxydopamine (6-OHDA)-stressed SH-SY5Y cells, known as an optimal cell for neurotoxic research. Levels of apoptotic markers were evaluated using quantitative polymerase chain reaction (qPCR) and flow cytometry. The localization of apoptotic cells in vivo was analyzed using whole-mount immunochemistry and the terminal deoxynucleo-tidyl transferase dUTP nick end labeling assay. Additionally, reactive oxygen species (ROS) pro-duction was estimated using flow cytometry. 6-OHDA induced ROS production in neuroblast cells and in vivo, but treatment with the extract protected against the 6-OHDA-induced increase in ROS levels. Under oxidative stress, the extract performs three protective functions: decreases ROS production, prevents mitochondrial apoptosis, and protects the central and ventral nervous systems. These results also suggest that the extract can be useful for the development of functional foods for the prevention of neural damage due to oxidative stress.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
