Version 1
: Received: 2 May 2024 / Approved: 3 May 2024 / Online: 3 May 2024 (07:17:22 CEST)
How to cite:
Sugasawa, T.; Hirokawa, A.; Otani, N.; Kanki, Y.; Nguyen, K. D.; Takemasa, T.; Watanabe, K.; Takeuchi, Y.; Yahagi, N.; Takahashi, Y. Detection Method for Gene Doping in a Mouse Model Expressing Human Erythropoietin from Adeno-Associated Virus Vector-9. Preprints2024, 2024050167. https://doi.org/10.20944/preprints202405.0167.v1
Sugasawa, T.; Hirokawa, A.; Otani, N.; Kanki, Y.; Nguyen, K. D.; Takemasa, T.; Watanabe, K.; Takeuchi, Y.; Yahagi, N.; Takahashi, Y. Detection Method for Gene Doping in a Mouse Model Expressing Human Erythropoietin from Adeno-Associated Virus Vector-9. Preprints 2024, 2024050167. https://doi.org/10.20944/preprints202405.0167.v1
Sugasawa, T.; Hirokawa, A.; Otani, N.; Kanki, Y.; Nguyen, K. D.; Takemasa, T.; Watanabe, K.; Takeuchi, Y.; Yahagi, N.; Takahashi, Y. Detection Method for Gene Doping in a Mouse Model Expressing Human Erythropoietin from Adeno-Associated Virus Vector-9. Preprints2024, 2024050167. https://doi.org/10.20944/preprints202405.0167.v1
APA Style
Sugasawa, T., Hirokawa, A., Otani, N., Kanki, Y., Nguyen, K. D., Takemasa, T., Watanabe, K., Takeuchi, Y., Yahagi, N., & Takahashi, Y. (2024). Detection Method for Gene Doping in a Mouse Model Expressing Human Erythropoietin from Adeno-Associated Virus Vector-9. Preprints. https://doi.org/10.20944/preprints202405.0167.v1
Chicago/Turabian Style
Sugasawa, T., Naoya Yahagi and Yoichiro Takahashi. 2024 "Detection Method for Gene Doping in a Mouse Model Expressing Human Erythropoietin from Adeno-Associated Virus Vector-9" Preprints. https://doi.org/10.20944/preprints202405.0167.v1
Abstract
With the rapid development of gene therapy technology in recent years, its abuse as a method of sports doping in athletic has become a concern. However, there is still room for improvement in gene doping testing methods, and a robust animal model needs to be developed. Therefore, the purposes of this study were to establish a model of gene doping using recombinant adeno-associated virus vector-9 including the human erythropoietin gene (rAAV9-hEPO), and to establish a relevant testing method. First, it was attempted to establish the model using rAAV9-hEPO on mice. The results showed a significant increase in erythrocyte volume accompanied by an increase in spleen weight, confirming the validity of the model. Next, we attempted to detect proof of gene doping by targeting DNA and RNA. Direct proof of gene doping was detected using a TaqMan-qPCR assay with certain primers/probes. In addition, some indirect proof was identified in RNAs through the combination of a TB Green qPCR assay with RNA sequencing. Taken together, these results could provide the foundation for an effective test for gene doping in human athletes in the future.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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