PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways
Version 1
: Received: 30 April 2024 / Approved: 30 April 2024 / Online: 30 April 2024 (17:07:59 CEST)
How to cite:
Lopes, F. B. T. P.; Schlatzer, D.; Li, M.; Yilmaz, S.; Wang, R.; Qi, X.; Ayati, M.; Koyuturk, M.; Chance, M. R. Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways. Preprints2024, 2024042007. https://doi.org/10.20944/preprints202404.2007.v1
Lopes, F. B. T. P.; Schlatzer, D.; Li, M.; Yilmaz, S.; Wang, R.; Qi, X.; Ayati, M.; Koyuturk, M.; Chance, M. R. Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways. Preprints 2024, 2024042007. https://doi.org/10.20944/preprints202404.2007.v1
Lopes, F. B. T. P.; Schlatzer, D.; Li, M.; Yilmaz, S.; Wang, R.; Qi, X.; Ayati, M.; Koyuturk, M.; Chance, M. R. Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways. Preprints2024, 2024042007. https://doi.org/10.20944/preprints202404.2007.v1
APA Style
Lopes, F. B. T. P., Schlatzer, D., Li, M., Yilmaz, S., Wang, R., Qi, X., Ayati, M., Koyuturk, M., & Chance, M. R. (2024). Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways. Preprints. https://doi.org/10.20944/preprints202404.2007.v1
Chicago/Turabian Style
Lopes, F. B. T. P., Mehmet Koyuturk and Mark R Chance. 2024 "Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways" Preprints. https://doi.org/10.20944/preprints202404.2007.v1
Abstract
Methionine oxidation to the sulfoxide form (MSox) is a poorly understood post-translational modification of proteins associated with nonspecific chemical oxidation from reactive oxygen species (ROS) whose chemistries are linked to various disease pathologies including neurodegeneration. Emerging evidence shows MSox site occupancy is in some cases under enzymatic regulatory control mediating cellular signaling including phosphorylation and/or calcium signaling, raising questions as to the speciation and functional nature of MSox across the proteome. The 5XFAD lineage of the C57BL/6 mouse has well-defined Alzheimer’s and aging states. Using this model, we analyzed age, sex and disease dependent MSox speciation in mouse hippocampus. In addition, we explore chemical stability and statistical variance of oxidized peptide signals to understand needed power for MSox based proteome studies. Our results identify mitochondrial and glycolytic pathway targets with increases in MSox with age as well as neuroinflammatory targets accumulating MSox with AD in proteome studies of mouse hippocampus. Further, the paper establishes a foundation for reproducible and rigorous experimental MSox-omics appropriate for biological discovery novel target identification, and biomarker analysis in ROS and other oxidation linked diseases.
Keywords
Methionine Oxidation; MSox; ROS; Proteomics; 5XFAD; Alzheimer’s Disease, mass spectrometry
Subject
Biology and Life Sciences, Biophysics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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