Version 1
: Received: 24 April 2024 / Approved: 25 April 2024 / Online: 25 April 2024 (16:10:13 CEST)
How to cite:
Silver, B.B.; Brooks, A.; Gerrish, K.; Tokar, E.J. Isolation and Characterization of Cell-Free DNA from Cerebral Organoids. Preprints2024, 2024041690. https://doi.org/10.20944/preprints202404.1690.v1
Silver, B.B.; Brooks, A.; Gerrish, K.; Tokar, E.J. Isolation and Characterization of Cell-Free DNA from Cerebral Organoids. Preprints 2024, 2024041690. https://doi.org/10.20944/preprints202404.1690.v1
Silver, B.B.; Brooks, A.; Gerrish, K.; Tokar, E.J. Isolation and Characterization of Cell-Free DNA from Cerebral Organoids. Preprints2024, 2024041690. https://doi.org/10.20944/preprints202404.1690.v1
APA Style
Silver, B.B., Brooks, A., Gerrish, K., & Tokar, E.J. (2024). Isolation and Characterization of Cell-Free DNA from Cerebral Organoids. Preprints. https://doi.org/10.20944/preprints202404.1690.v1
Chicago/Turabian Style
Silver, B.B., Kevin Gerrish and Erik J Tokar. 2024 "Isolation and Characterization of Cell-Free DNA from Cerebral Organoids" Preprints. https://doi.org/10.20944/preprints202404.1690.v1
Abstract
Early detection of neurological conditions is critical for timely diagnosis and treatment. Identifying cellular-level changes is essential for implementing therapeutic interventions prior to symptomatic disease onset. However, monitoring brain tissue directly through biopsies is invasive and high-risk. Bodily fluids such as blood or cerebrospinal fluid contain information in many forms, including proteins and nucleic acids. Particularly, cell-free DNA (cfDNA) has potential as a versatile neurological biomarker. Yet, our knowledge of cfDNA released by brain tissue and how cfDNA changes in response to deleterious events within the brain is incomplete. Mapping changes in cfDNA to specific cellular events is difficult in vivo, where many tissues contribute to circulating cfDNA. Organoids are tractable systems for examining specific changes consistently in a human background. However, few studies have investigated cfDNA released from organoids. Here, we examined cfDNA isolated from cerebral organoids. We found that cerebral organoids release quantities of cfDNA sufficient for downstream analysis with droplet-digital PCR and whole-genome sequencing. Further, gene ontology analysis of genes aligning with sequenced cfDNA fragments revealed association with terms related to neurodevelopment and autism spectrum disorder. We conclude that cerebral organoids hold promise as tools for the discovery of cfDNA biomarkers related to neurodevelopmental and neurological disorders.
Keywords
biomarkers; cell-free DNA; cerebral organoid; new approach methodologies; liquid biopsy
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
