PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex
Version 1
: Received: 18 April 2024 / Approved: 18 April 2024 / Online: 18 April 2024 (13:38:21 CEST)
How to cite:
Murphy, D.; Barker, N.P.; Frisby, A. Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints2024, 2024041263. https://doi.org/10.20944/preprints202404.1263.v1
Murphy, D.; Barker, N.P.; Frisby, A. Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints 2024, 2024041263. https://doi.org/10.20944/preprints202404.1263.v1
Murphy, D.; Barker, N.P.; Frisby, A. Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints2024, 2024041263. https://doi.org/10.20944/preprints202404.1263.v1
APA Style
Murphy, D., Barker, N.P., & Frisby, A. (2024). Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints. https://doi.org/10.20944/preprints202404.1263.v1
Chicago/Turabian Style
Murphy, D., Nigel Paul Barker and Arnold Frisby. 2024 "Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex" Preprints. https://doi.org/10.20944/preprints202404.1263.v1
Abstract
Recent technological advancements in conservation genetics and genomics have resulted in diverse tools for aiding conservation of species. The precision and resolution of high throughput sequencing technologies provide valuable insights to aid conservation decisions, but these technologies are often financially unfeasible or unavailable in resource constrained countries. Inter-Simple Sequence Repeat (ISSR) markers, when combined with sensitive automated detection systems, provide a simple, cheap means to investigate genetic diversity and discriminate closely related species. Here we apply this technology to assess genetic diversity and taxonomic delimitation in the Encephalartos eugene-maraisii species complex, a highly threatened, taxonomically dubious group of cycads in South Africa. Silica dried cycad leaflets were extracted using the Oktopure robot employing Sbeadex chemistry and amplified with fluorescently tagged ISSR primers. Band presence was scored with an ABI 3130 genetic sequencer under three different fluorescence cut-off values. Phenetic analyses were performed in NTSYS software while Bayesian analysis was performed in STRUCTURE, and haplotype network analysis, AMOVA and Tajima’s D statistic was computed with PopART. Our analyses support taxonomic singularity of E. dyerianus, E. dolomiticus and E. eugene-maraisii. Relationships between E. nubimontanus and E. cupidus remain uncertain. E. middelburgensis samples showed no clustering but had poor amplification success. This study demonstrated the suitability of automated ISSR fingerprinting as a method for plant conservation studies, especially in resource-constrained countries.
Keywords
cycad; Inter-Simple Sequence Repeats; conservation genetics; species complex, Critically Endangered
Subject
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
