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Submitted:
13 April 2024
Posted:
15 April 2024
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Instrument | Model and manufacturer |
---|---|
Autoclaves | Tomy, japan |
Aerobic incubator | Sanyo, Japan |
Digital balance | Mettler Toledo, Switzerland |
Oven | Binder, Germany |
Deep freezer -70 0C | Artiko |
Refrigerator 5 | whirlpool |
PH meter electrode | Mettler-toledo,UK |
Deep freezer -20 0C | whirlpool |
Gyrator shaker | Corning gyrator shaker, Japan |
190-1100nm Ultraviolet visible spectrophotometer | UV1600PC, China |
Light(optical) microscope | Amscope 120X-1200X, China |
Pathogenic m.o | No of strains | Isolation media |
---|---|---|
Bacillus subtilis | 5 | Mannitol egg yolk polymixin agar( MEYP) |
Bacillus cereus | 7 | Polymixin egg yolk mannitol bromothymol blue agar( PEMBA) |
Staphylococcus aureus | 6 | Salt mannitol agar( SMA) |
Pneumococci | 13 | Todd Hewitt broth with yeast extract |
coli | 17 | Sorbitol- Macconkey agar |
Pseudomonas aeruginosa | 10 | Pseudomonas isolation agar( PSA) |
Candida albicans | 1 | Potato dextrose agar( PDA) |
Saccharomyces cerevisiae | 5 | Sabourad dextrose agar( SDA) |
Salmonella typhimurium | 4 | Bismuth sulfite agar( BSA) |
Haemophilus influenza | 3 | Enriched chocolate agar |
Gonococci | 4 | Thayer martin medium |
Meningococci | 6 | Mueller Hinton agar |
Serratia Marcescens | 4 | Caprylate thallous agar medium |
Mucor hiemalis | 1 | Potato dextrose broth |
Shigella dysenteriae | 8 | Hekteon enteric agar |
Micrococcus luteus | 1 | Tryptic soy agar |
Proteus mirabilis | 1 | Blood agar |
No of +ve bacterial isolates producing Myxopyronin A | No of -ve bacterial isolates producing Myxopyronin A |
19 | 31 |
Test antibiotic | Degree of purity( %) |
---|---|
Myxopyronin A | 80 |
Myxopyronin B | 20 |
Description | Scientific Name | Max Score | Total Score | Query Cover | E value | Per. ident |
Myxococcus fulvus 124B02, complete genome | Myxococcus fulvus 124B02 | 597 | 1167 | 99% | 6.00E-166 | 98.81 |
Myxococcus sp. MH1 DNA, complete genome | Myxococcus sp. MH1 | 525 | 1051 | 99% | 3.00E-144 | 94.94 |
Myxococcus sp. SDU36 chromosome, complete genome | Myxococcus sp. SDU36 | 436 | 870 | 99% | 1.00E-117 | 90.15 |
Myxococcus hansupus strain mixupus chromosome, complete genome | Myxococcus hansupus | 126 | 126 | 65% | 3.00E-24 | 77.23 |
Cystobacter fuscus strain DSM 52655 chromosome, complete genome | Cystobacter fuscus | 124 | 124 | 54% | 1.00E-23 | 78.92 |
Cystobacter fuscus strain Cbf 8 chromosome, complete genome | Cystobacter fuscus | 124 | 124 | 54% | 1.00E-23 | 78.92 |
Test organism | MIC( µg/ ml) | Diameter of inhibition zone( mm) |
---|---|---|
Bacillus subtilis | 5 | 12 |
Staphylococcus aureus | 7 | 18 |
Streptococcus pneumonae | 13 | 9 |
Escherichia coli | 108 | 11 |
Pseudomonas aeruginosa | 120 | 0 |
Candida albicans | 105 | 0 |
Sacchromyces cerevisiae | 100 | 0 |
Salmonella typhimurium | 101 | 17 |
Bacillus cereus | 16 | 10 |
Micrococcus luteus | 21 | 13 |
Serratia Marcescens | 105 | 9 |
Mucor hiemalis | 0 | 19 |
Shigella dysentery | 113 | 7 |
Proteus mirabilis | 139 | 6 |
Pathogenic m.o | MIC( µg/ ml) |
---|---|
Bacillus subtilis | 8 |
Bacillus cereus | 7 |
Staphylococcus aureus | 13 |
Pneumococci | 15 |
E.coli | 110 |
Pseudomonas aeruginosa | 142 |
Candida albicans | 0 |
Sacchromyces cerevisiae | 0 |
Salmonella typhimurium | 103 |
Haemophilus influenza | 0 |
Gonococci | 127 |
meningococci | 134 |
Serratia Marcescens | 105 |
Mucor hiemalis | 0 |
Shigella dysenteriae | 113 |
Micrococcus luteus | 0 |
Proteus mirabilis | 0 |
Pathogenic m.o | MBC( µg/ ml) |
---|---|
Bacillus subtilis | 24 |
Bacillus cereus | 21 |
Staphylococcus aureus | 39 |
Pneumococci | 46 |
E.coli | 331 |
Pseudomonas aeruginosa | 412 |
Candida albicans | 0 |
Sacchromyces cerevisiae | 0 |
Salmonella typhimurium | 307 |
Haemophilus influenza | 0 |
Gonococci | 370 |
meningococci | 390 |
Serratia Marcescens | 320 |
Mucor hiemalis | 0 |
Shigella dysenteriae | 303 |
Micrococcus luteus | 0 |
Proteus mirabilis | 0 |
mRNA concentration( ng/ ml) | Absorbance( optical density) at 260 nm |
---|---|
810 | 0.973 |
705 | 0.601 |
341 | 0.397 |
43 | 0.213 |
Bacterial protein concentration( mcg/ ml) | Time( hr) |
---|---|
70.3 | 1 |
41.06 | 3 |
18.62 | 5 |
3.01 | 10 |
0.79 | 12 |
Test | Result |
---|---|
Gram stain | - |
Cell shape | Elongated bacilli with tapered ends |
Spore shape | Ellipsoidal |
Spore site | Central |
Motility | + via gliding |
Catalase | + |
Oxidase | - |
Blood haemolysis | - |
Indol | - |
Methyl red | - |
Nitrate reduction test | + |
Vogues proscauer | - |
Citrate utilization | - |
Starch hydrolysis | + |
Casein hydrolysis | + |
Growth at 45 ℃ | Bacterial isolates did not grow at 45 ℃; but were grown at 10-37 ℃ |
Tween 80 | + |
Tolerance salinity | |
5% NaCl | - |
7% NaCl | - |
Saccharide fermentation | |
Glucose | - |
Fructose | - |
Maltose | - |
Sucrose | - |
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