Version 1
: Received: 12 April 2024 / Approved: 12 April 2024 / Online: 12 April 2024 (08:00:05 CEST)
How to cite:
Wen, Y.; Huang, S.; Lei, H.; Li, X.; Shen, X. A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle-Derived and Soybean-Derived Adulteration in Goat Milk Powder. Preprints2024, 2024040839. https://doi.org/10.20944/preprints202404.0839.v1
Wen, Y.; Huang, S.; Lei, H.; Li, X.; Shen, X. A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle-Derived and Soybean-Derived Adulteration in Goat Milk Powder. Preprints 2024, 2024040839. https://doi.org/10.20944/preprints202404.0839.v1
Wen, Y.; Huang, S.; Lei, H.; Li, X.; Shen, X. A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle-Derived and Soybean-Derived Adulteration in Goat Milk Powder. Preprints2024, 2024040839. https://doi.org/10.20944/preprints202404.0839.v1
APA Style
Wen, Y., Huang, S., Lei, H., Li, X., & Shen, X. (2024). A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle-Derived and Soybean-Derived Adulteration in Goat Milk Powder. Preprints. https://doi.org/10.20944/preprints202404.0839.v1
Chicago/Turabian Style
Wen, Y., Xiangmei Li and Xing Shen. 2024 "A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle-Derived and Soybean-Derived Adulteration in Goat Milk Powder" Preprints. https://doi.org/10.20944/preprints202404.0839.v1
Abstract
The adulteration of goat milk powder occurs frequently, cattle-derived and soybean-derived ingredients are common adulterants in goat milk powder. However, simultaneously and rapidly detecting cattle-derived and soybean-derived components is still a challenge. high throughput and efficient screening method for adulteration detection is need. In this study, a rapid method was developed to detect the adulteration of common cattle-derived and soybean-derived components simultaneously in goat milk powder by combining the CRISPR/ Cas12a system with recombinant polymerase amplification (RPA). A dual DNA extraction method was employed, primers and crRNA for dual detection were designed and screened, and a series of condition optimizations were carried out in this experiment. The optimized assay can rapidly detect cattle-derived and soybean-derived components in 40 min. The detection limits of both cattle-derived and soybean-derived components were 1% (w/w) for the mixed adulteration models. The established method was applied into a blind survey of 55 commercially available goat milk powder products. The results showed that 36.36% of the samples contained cattle-derived or soybean-derived ingredients, which revealed the noticeable adulteration situation in the goat milk powder market. This study realizes a fast flow of dual extraction, dual amplification and dual detection of cattle-derived and soybean-derived components in goat milk powder for the first time. It can be used for high-throughput and high-efficiency on-site primary screening of goat milk powder adulterants, and provide a technical reference for combating with food adulteration.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
