Version 1
: Received: 1 April 2024 / Approved: 1 April 2024 / Online: 1 April 2024 (14:56:36 CEST)
How to cite:
Wendlandt, T.; Britz, B.; Kleinow, T.; Hipp, K.; Eber, F.J.; Wege, C. Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach. Preprints2024, 2024040107. https://doi.org/10.20944/preprints202404.0107.v1
Wendlandt, T.; Britz, B.; Kleinow, T.; Hipp, K.; Eber, F.J.; Wege, C. Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach. Preprints 2024, 2024040107. https://doi.org/10.20944/preprints202404.0107.v1
Wendlandt, T.; Britz, B.; Kleinow, T.; Hipp, K.; Eber, F.J.; Wege, C. Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach. Preprints2024, 2024040107. https://doi.org/10.20944/preprints202404.0107.v1
APA Style
Wendlandt, T., Britz, B., Kleinow, T., Hipp, K., Eber, F.J., & Wege, C. (2024). Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach. Preprints. https://doi.org/10.20944/preprints202404.0107.v1
Chicago/Turabian Style
Wendlandt, T., Fabian J. Eber and Christina Wege. 2024 "Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach" Preprints. https://doi.org/10.20944/preprints202404.0107.v1
Abstract
This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages, and presents a novel efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions.
We survey the various driving forces prompting researchers to enrich tobamoviruses: from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. Best practice and also rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls and 'forgotten' or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of the multivalent soft-matter nanorods.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
