Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In vitro Effects of Postmetabolites from Limosilactobacillus fermentum 53 on the Survival and Proliferation of HT-29 Cells

Veselina Svetoslavova Moskova-Doumanova
ORCID logo ,
Anita Stoyanova Vaseva
,
Ralitsa Krumcheva Veleva
,
Kirilka Stefanova Mladenova
,
Denitsa Ruenova Melnishka
,
Jordan Doumanov
,
Pavel Veselinov Videv
,
Tanya Ivanova Topouzova-Hristova
ORCID logo ,
Lili Ivailova Dobreva
,
Nikoleta Trifonova Atanasova
ORCID logo ,
Svetla Trifonova Danova
*
Version 1 : Received: 29 March 2024 / Approved: 1 April 2024 / Online: 1 April 2024 (12:38:51 CEST)

How to cite: Moskova-Doumanova, V.S.; Vaseva, A.S.; Veleva, R.K.; Mladenova, K.S.; Melnishka, D.R.; Doumanov, J.; Videv, P.V.; Topouzova-Hristova, T.I.; Dobreva, L.I.; Atanasova, N.T.; Danova, S.T. In vitro Effects of Postmetabolites from Limosilactobacillus fermentum 53 on the Survival and Proliferation of HT-29 Cells. Preprints 2024, 2024040048. https://doi.org/10.20944/preprints202404.0048.v1 Moskova-Doumanova, V.S.; Vaseva, A.S.; Veleva, R.K.; Mladenova, K.S.; Melnishka, D.R.; Doumanov, J.; Videv, P.V.; Topouzova-Hristova, T.I.; Dobreva, L.I.; Atanasova, N.T.; Danova, S.T. In vitro Effects of Postmetabolites from Limosilactobacillus fermentum 53 on the Survival and Proliferation of HT-29 Cells. Preprints 2024, 2024040048. https://doi.org/10.20944/preprints202404.0048.v1

Abstract

Naturally fermented dairy products are an important component of the human diet. They are a valuable source of nutrients as well as vitamins and minerals. Their importance as a source of probiotic bacterial strains should not be overlooked. A number of studies highlights the positive effects of species of the Lactobacillacea on the intestinal microbiota, the overall homeostasis of the body, as well as a complementary treatment for some diseases. However, data on the effects on intestinal epithelial cells of postmetabolites released by bacteria are incomplete. This is likely due to the fact that these effects are species and strain specific. In the present study, we investigated the effects of postmetabolites produced by a pre-selected candidate probiotic strain Limosilactobacillus fermentum on HT-29 intestinal epithelial cells. Our data showed a pronounced proliferative effect, evaluated by flow cytometry, quantification of cell population, and determination of mitotic index. This was accompanied by stabilization of the cell monolayer, measured by increase of TEER (trans epithelial electric resistance) and reorganization of actin filaments. The data obtained are a clear indication of the positive effects, that the products secreted by L. fermentum strain 53 have on the intestinal epithelial cells.

Keywords

Limosilactobacillus fermentum;  TEER; postmetabolites; HT-29 cells 

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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