A High-Homology Region in Bacterial β-Barrel Pore-Forming Toxins

How to cite: Nagel, A.S.; Vetrova, O.; Rudenko, N.V.; Karatovskaya, A.P.; Zamyatina, A.; Andreeva-Kovalevskaya, Z.I.; Salyamov, V.; Egorova, N.; Siunov, A.V.; Ivanova, T.D.; Boziev, K.M.; Brovko, F.A.; Solonin, A.S. A High-Homology Region in Bacterial β-Barrel Pore-Forming Toxins. Preprints 2024, 2024031156. https://doi.org/10.20944/preprints202403.1156.v1 Nagel, A.S.; Vetrova, O.; Rudenko, N.V.; Karatovskaya, A.P.; Zamyatina, A.; Andreeva-Kovalevskaya, Z.I.; Salyamov, V.; Egorova, N.; Siunov, A.V.; Ivanova, T.D.; Boziev, K.M.; Brovko, F.A.; Solonin, A.S. A High-Homology Region in Bacterial β-Barrel Pore-Forming Toxins. Preprints 2024, 2024031156. https://doi.org/10.20944/preprints202403.1156.v1

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MDPI and ACS Style

Nagel, A.S.; Vetrova, O.; Rudenko, N.V.; Karatovskaya, A.P.; Zamyatina, A.; Andreeva-Kovalevskaya, Z.I.; Salyamov, V.; Egorova, N.; Siunov, A.V.; Ivanova, T.D.; Boziev, K.M.; Brovko, F.A.; Solonin, A.S. A High-Homology Region in Bacterial β-Barrel Pore-Forming Toxins. Preprints 2024, 2024031156. https://doi.org/10.20944/preprints202403.1156.v1

APA Style

Nagel, A.S., Vetrova, O., Rudenko, N.V., Karatovskaya, A.P., Zamyatina, A., Andreeva-Kovalevskaya, Z.I., Salyamov, V., Egorova, N., Siunov, A.V., Ivanova, T.D., Boziev, K.M., Brovko, F.A., & Solonin, A.S. (2024). A High-Homology Region in Bacterial β-Barrel Pore-Forming Toxins. Preprints. https://doi.org/10.20944/preprints202403.1156.v1

Chicago/Turabian Style

Nagel, A.S., Fedor A. Brovko and Alexander S. Solonin. 2024 "A High-Homology Region in Bacterial β-Barrel Pore-Forming Toxins" Preprints. https://doi.org/10.20944/preprints202403.1156.v1

Abstract

Pathogenicity of many bacteria including Bacillus cereus and Staphylococcus aureus depends on pore-forming toxins (PFTs), which cause lysis of host cells by forming pores in membranes of eukaryotic cells. Bioinformatic analysis revealed in over 600 PFTs a region homologous to the Lys171-Gly250 sequence in hemolysin II (HlyII) from B. cereus, which we designated as “homologous peptide”. Three β-barrel PFTs were used for a detailed comparative analysis. Two of them –HlyII and cytotoxin K2 (CytK2), – are synthesized in Bacillus cereus sensu lato; the third – S. aureus α-toxin (Hla) – is the most investigated representative of the family. Protein modeling showed certain amino acids of homologous peptide to be located on the surface of the monomeric forms of these β-barrel PFTs. We obtained monoclonal antibodies against both a cloned homologous peptide and a 14-membered synthetic peptide DSFNTFYGNQLFMK as part of homologous peptide. The HlyII, CytK2 and Hla regions recognized by the obtained antibodies, as well as an antibody capable of suppressing the hemolytic activity of CytK2, were identified in the course of the work. Antibodies capable of recognizing PFTs of various origins can be useful tools for both identification and suppression of the cytolytic activity of PFTs.

Keywords

Staphylococcus aureus α-toxin; Bacillus cereus hemolysin II; cytotoxin K2; suppression of hemolytic activity; monoclonal antibodies; enzyme immunoassay

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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