Version 1
: Received: 8 March 2024 / Approved: 8 March 2024 / Online: 8 March 2024 (14:40:56 CET)
How to cite:
Zhou, X.; Zhang, X.; Wang, D.; Luo, R.; Qin, Z.; Li, W.; Liu, X.; Lin, F.; Xia, X.; Hu, G. Efficient Biosynthesis of Salidroside via Artificial In Vivo Enhanced UDP-Glucose System Using Cheap Sucrose as Substrate in Saccharomyces cerevisiae. Preprints2024, 2024030533. https://doi.org/10.20944/preprints202403.0533.v1
Zhou, X.; Zhang, X.; Wang, D.; Luo, R.; Qin, Z.; Li, W.; Liu, X.; Lin, F.; Xia, X.; Hu, G. Efficient Biosynthesis of Salidroside via Artificial In Vivo Enhanced UDP-Glucose System Using Cheap Sucrose as Substrate in Saccharomyces cerevisiae. Preprints 2024, 2024030533. https://doi.org/10.20944/preprints202403.0533.v1
Zhou, X.; Zhang, X.; Wang, D.; Luo, R.; Qin, Z.; Li, W.; Liu, X.; Lin, F.; Xia, X.; Hu, G. Efficient Biosynthesis of Salidroside via Artificial In Vivo Enhanced UDP-Glucose System Using Cheap Sucrose as Substrate in Saccharomyces cerevisiae. Preprints2024, 2024030533. https://doi.org/10.20944/preprints202403.0533.v1
APA Style
Zhou, X., Zhang, X., Wang, D., Luo, R., Qin, Z., Li, W., Liu, X., Lin, F., Xia, X., & Hu, G. (2024). Efficient Biosynthesis of Salidroside via Artificial In Vivo Enhanced UDP-Glucose System Using Cheap Sucrose as Substrate in Saccharomyces cerevisiae. Preprints. https://doi.org/10.20944/preprints202403.0533.v1
Chicago/Turabian Style
Zhou, X., Xue Xia and Ge Hu. 2024 "Efficient Biosynthesis of Salidroside via Artificial In Vivo Enhanced UDP-Glucose System Using Cheap Sucrose as Substrate in Saccharomyces cerevisiae" Preprints. https://doi.org/10.20944/preprints202403.0533.v1
Abstract
Salidroside, a valuable phenylethanoid glycoside, is obtained from plants belonging to the Rhodiola genus, known for its diverse biological properties. At present, salidroside is still far from large-scale industrial production due to the lower titer and higher process cost. In this study, we constructed a biosynthetic pathway from L-tyrosine to salidroside in Saccharomyces cerevisiae. To increase sali-droside production, we for the first time constructed an in vivo UDP-glucose regeneration system that sucrose synthase GmSUS worked in conjunction with UDP-glucose transferase RrUGT33 to improve the UDP-glucose availability with inexpensive sucrose as the consumed substrate. Finally, the molar conversion rate of L-tyrosine could reach 95%. To further reduce the cost, co-culture with E. coli that synthesized L-tyrosine de novo resulted in an 81% reduction in the final substrate cost per 1 kg of salidroside compared to the initial cost. The final titer of salidroside obtained 3.8 g/L and was the highest titer achieved at the Shake-Flask-Level in the existing reports. And this marked the first successful synthesis of salidroside in an in situ enhanced UDP-glucose system using sucrose. This accomplishment laid a robust groundwork for further investigations into the synthesis of other notable glycosides and natural compounds.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
