Version 1
: Received: 8 March 2024 / Approved: 8 March 2024 / Online: 8 March 2024 (12:57:36 CET)
How to cite:
Pintão, A.M.; Santos, T.A.P.; Nogueira, F. Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate. Preprints2024, 2024030530. https://doi.org/10.20944/preprints202403.0530.v1
Pintão, A.M.; Santos, T.A.P.; Nogueira, F. Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate. Preprints 2024, 2024030530. https://doi.org/10.20944/preprints202403.0530.v1
Pintão, A.M.; Santos, T.A.P.; Nogueira, F. Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate. Preprints2024, 2024030530. https://doi.org/10.20944/preprints202403.0530.v1
APA Style
Pintão, A.M., Santos, T.A.P., & Nogueira, F. (2024). Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate. Preprints. https://doi.org/10.20944/preprints202403.0530.v1
Chicago/Turabian Style
Pintão, A.M., Tiago Alexandre Pena Santos and Fátima Nogueira. 2024 "Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate" Preprints. https://doi.org/10.20944/preprints202403.0530.v1
Abstract
Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of BITC and T. majus seeds, leaves, and stems aqueous extracts. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of chloroquine-susceptible Plasmodium falciparum (P. falciparum) 3D7-GFP strain. Extracts and/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7-GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity higher in seeds than in leaves or stems. BITC’s GI was comparable to chloroquine’s. BITC’s IC50 was similar in both strains, thus a cross-resistance absence with aminoquinolines was found (RI < 1). BITC presented features that could open new avenues for malaria drug discovery.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
