AFM Study of the Influence of Stopped Glycerol Flow in a Coil on Peroxidase Enzyme

Yuri D. Ivanov; Ivan D. Shumov; Andrey F. Kozlov; Anastasia A. Valueva; Maria O. Ershova; Irina A. Ivanova; Alexander N. Ableev; Vadim Y. Tatur; Andrei A. Lukyanitsa; Nina D. Ivanova; Vadim S. Ziborov

doi:10.20944/preprints202403.0304.v1

Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 4 March 2024 / Approved: 6 March 2024 / Online: 6 March 2024 (10:56:45 CET)

Glycerol represents a useful functional component of heat-transfer fluids. Glycerol is known to be triboelectrically active. We report how the flow of glycerol, stopped in a cylindrical coiled pipe, influences horseradish peroxidase (HRP) enzyme. Namely, glycerol flow was pumped through the coil and then stopped. After that, we incubated the 0.1 µM solution of HRP near the side of the coil, and then adsorbed HRP onto mica. This operation markedly increased the HRP aggregation on mica in comparison with the control sample. We explain this phenomenon by the influence of triboelectrically induced electromagnetic fields. Our results can be useful in the development of flow-based systems for biosensors and bioreactors.

horseradish peroxidase; glycerol; atomic force microscopy; enzyme aggregation

Biology and Life Sciences, Biophysics

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AFM Study of the Influence of Stopped Glycerol Flow in a Coil on Peroxidase Enzyme

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