Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biological Monitoring of Extracorporeal Photopheresis for Steroid Resistant Acute Graft versus Host Disease by a Short Culture Method and Flow Cytometry

Luca Pierelli
* ORCID logo ,
Anna Capalbo
,
Eleonora Ponte
,
Paola Iudicone
,
Stefano Spurio
,
Sara Morara
,
Roberta Battistini
,
Anna Proia
Version 1 : Received: 23 February 2024 / Approved: 23 February 2024 / Online: 26 February 2024 (09:40:12 CET)

How to cite: Pierelli, L.; Capalbo, A.; Ponte, E.; Iudicone, P.; Spurio, S.; Morara, S.; Battistini, R.; Proia, A. Biological Monitoring of Extracorporeal Photopheresis for Steroid Resistant Acute Graft versus Host Disease by a Short Culture Method and Flow Cytometry. Preprints 2024, 2024021399. https://doi.org/10.20944/preprints202402.1399.v1 Pierelli, L.; Capalbo, A.; Ponte, E.; Iudicone, P.; Spurio, S.; Morara, S.; Battistini, R.; Proia, A. Biological Monitoring of Extracorporeal Photopheresis for Steroid Resistant Acute Graft versus Host Disease by a Short Culture Method and Flow Cytometry. Preprints 2024, 2024021399. https://doi.org/10.20944/preprints202402.1399.v1

Abstract

Acute and chronic graft versus host disease (a/cGVHD) are major complications of allogeneic stem cell transplantation and a frequent cause of non relapse-mortality. The treatment of a/cGVHD consists of steroid administration at full dose. In the case of aGVHD, around 60 % of patients with grade III/IV fails to respond (steroid-resistant, SR), requiring a second line therapy. Extracorporeal photopheresis (ECP) represents a good option for second line treatment, leading to multiorgan response in several patients. Here, we have evaluated the performances of a cytofluorimetric quality control of the first ECP procedure in 29 patients with severe SR aGVHD who underwent ECP. The quality control represents a check control of in vitro cell apoptosis/death induction in collected and irradiated CD3+ cells by ECP. Our analysis shows that in more than 70 % quality controls a relevant apoptotosis/death of CD3+ cells was observed after cell irradiation, as compared to non-irradiated controls. Albeit observed in a small patients’ series, the occurrence of relevant in vitro effect after ECP relates to less procedures and/or to a better survival on ECP. Age, disease, gender, aGVHD grade and time from transplant to the first ECP had not impact on ECP efficacy in this patients’ series.

Keywords

graft versus host disease; extracorporeal photopheresis; T cell death; biological quality control

Subject

Medicine and Pharmacology, Hematology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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