Version 1
: Received: 21 February 2024 / Approved: 21 February 2024 / Online: 21 February 2024 (15:34:31 CET)
How to cite:
Pedarpour, S.; Zarei, S.; Mahmoodi, Z.; Ghafouri, H. The Combined Effects of Doxorubicin and a Novel Sulfonamide Derivative on Inducing Apoptosis in a Breast Cancer Cell Line (MCF-7). Preprints2024, 2024021233. https://doi.org/10.20944/preprints202402.1233.v1
Pedarpour, S.; Zarei, S.; Mahmoodi, Z.; Ghafouri, H. The Combined Effects of Doxorubicin and a Novel Sulfonamide Derivative on Inducing Apoptosis in a Breast Cancer Cell Line (MCF-7). Preprints 2024, 2024021233. https://doi.org/10.20944/preprints202402.1233.v1
Pedarpour, S.; Zarei, S.; Mahmoodi, Z.; Ghafouri, H. The Combined Effects of Doxorubicin and a Novel Sulfonamide Derivative on Inducing Apoptosis in a Breast Cancer Cell Line (MCF-7). Preprints2024, 2024021233. https://doi.org/10.20944/preprints202402.1233.v1
APA Style
Pedarpour, S., Zarei, S., Mahmoodi, Z., & Ghafouri, H. (2024). The Combined Effects of Doxorubicin and a Novel Sulfonamide Derivative on Inducing Apoptosis in a Breast Cancer Cell Line (MCF-7). Preprints. https://doi.org/10.20944/preprints202402.1233.v1
Chicago/Turabian Style
Pedarpour, S., Zeinab Mahmoodi and Hossein Ghafouri. 2024 "The Combined Effects of Doxorubicin and a Novel Sulfonamide Derivative on Inducing Apoptosis in a Breast Cancer Cell Line (MCF-7)" Preprints. https://doi.org/10.20944/preprints202402.1233.v1
Abstract
Chemical combinations are believed to reduce drug toxicity, delay cancer cell growth, and achieve enhanced efficacy compared with single active drugs. This research investigated the synergistic effect of DOX and Zm-093, a new sulfonamide derivative, on improving the potential for inducing apoptosis in breast cancer (BC) cell lines. First, the Zm-093 compound was synthesized and its structure was confirmed by FT-IR and NMR. Then, the IC50 values for DOX and Zm-093 were calculated to be 1.21 and 51.24 μM, respectively. To investigate the synergistic effect, Compusyn software was utilized to calculate the combination index (CI). Different methods, including western blotting, flow cytometry, and TUNEL, were used to evaluate the potential synergistic effects of these compounds on inducing apoptosis in MCF-7 cells. Combining the indexes obtained at all concentrations revealed synergistic results. The proapoptotic proteins Bax, tBid, and caspase-3, however, increased 2.4, 3.3, and 5.7 times more than those in the control group. Flow cytometry analysis and the TUNEL method in cells treated with DOX and Zm-093 showed that apoptosis was significantly higher than in other groups. The results confirmed the synergistic effect of DOX and ZM-093 on apoptotic factors expression in MCF-7 cells.
Keywords
Apoptosis; Breast cancer; Drug synergism; Doxorubicin; Sulfonamide
Subject
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
