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Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for Detection of SARS-CoV-2 S Protein on the Surface of Cold Chain Food Packaging
Li, X.; Wang, J.; Yang, G.; Fang, X.; Zhao, L.; Luo, Z.; Dong, Y. The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging. Molecules2024, 29, 1776.
Li, X.; Wang, J.; Yang, G.; Fang, X.; Zhao, L.; Luo, Z.; Dong, Y. The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging. Molecules 2024, 29, 1776.
Li, X.; Wang, J.; Yang, G.; Fang, X.; Zhao, L.; Luo, Z.; Dong, Y. The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging. Molecules2024, 29, 1776.
Li, X.; Wang, J.; Yang, G.; Fang, X.; Zhao, L.; Luo, Z.; Dong, Y. The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging. Molecules 2024, 29, 1776.
Abstract
The COVID-19 pandemic over the past years has shown a great need for rapid, low-cost and on-site detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, an aptamer-based colloidal gold nanoparticle lateral flow test strip was well developed to realize the visual detection of wild type SARS-CoV-2 spike protein (SP) and multiple variants. Under the optimal reaction conditions, a low detection limit of SARS-CoV-2 S protein of 0.68 nM was acquired, and the actual detection recovery was 83.3% to 108.8% for real world samples. This suggests a potential tool for the prompt detection of SARS-CoV-2 with good sensitivity and accuracy, and a new method for the development of alternative antibody test strips for the detection of other viral targets.
Keywords
SARS-CoV-2; S protein; COVID-19; lateral flow test strips; AuNPs; aptamer
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
