Version 1
: Received: 17 February 2024 / Approved: 18 February 2024 / Online: 19 February 2024 (10:11:32 CET)
How to cite:
Rattajak, P.; Aroonkesorn, A.; Smythe, C.; Wititsuwannakul, R.; Pitakpornpreecha, T. Suppressive Effects of L-quebrachitol on Osteoclast Formation and Activity through the Inhibition of the RANK Signalling Pathway. Preprints2024, 2024020926. https://doi.org/10.20944/preprints202402.0926.v1
Rattajak, P.; Aroonkesorn, A.; Smythe, C.; Wititsuwannakul, R.; Pitakpornpreecha, T. Suppressive Effects of L-quebrachitol on Osteoclast Formation and Activity through the Inhibition of the RANK Signalling Pathway. Preprints 2024, 2024020926. https://doi.org/10.20944/preprints202402.0926.v1
Rattajak, P.; Aroonkesorn, A.; Smythe, C.; Wititsuwannakul, R.; Pitakpornpreecha, T. Suppressive Effects of L-quebrachitol on Osteoclast Formation and Activity through the Inhibition of the RANK Signalling Pathway. Preprints2024, 2024020926. https://doi.org/10.20944/preprints202402.0926.v1
APA Style
Rattajak, P., Aroonkesorn, A., Smythe, C., Wititsuwannakul, R., & Pitakpornpreecha, T. (2024). Suppressive Effects of L-quebrachitol on Osteoclast Formation and Activity through the Inhibition of the RANK Signalling Pathway. Preprints. https://doi.org/10.20944/preprints202402.0926.v1
Chicago/Turabian Style
Rattajak, P., Rapepun Wititsuwannakul and Thanawat Pitakpornpreecha. 2024 "Suppressive Effects of L-quebrachitol on Osteoclast Formation and Activity through the Inhibition of the RANK Signalling Pathway" Preprints. https://doi.org/10.20944/preprints202402.0926.v1
Abstract
Inositol is a natural carbocyclic sugar that plays an essential role in regulating the vital cellular functions of plants and animals. Current research has explored methyl derivatives of inositol, reporting on their various biological activities, including antitumor, anti-inflammatory, and anti-osteoporosis activities. Our previous study demonstrated that L-quebrachitol, a methyl derivative of inositol, enhances osteoblastogenesis and bone formation; however, its effect on osteoclastogenesis remains unclear. Consequently, we aimed to investigate the effect of L-quebrachitol on the receptor activator of nuclear factor-κB ligand-induced osteoclastogenesis in pre-osteoclastic RAW 264.7 cells. The results revealed that L-quebrachitol suppressed RANK-mediated signalling, including nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and Fos proto-oncogene (cFOS) pathways, at both gene and protein levels. Moreover, the critical transcription factor for osteoclastogenesis, nuclear factor of activated T cells c1 (NFATc1), was downregulated. Inhibition of osteoclast-associated marker genes encoding proteolytic enzymes, such as tartrate-resistant acid phosphatase (TRAP), matrix metallopeptidase 9 (MMP-9), and cathepsin K, led to reduced formation of TRAP-positive multinucleated cells and resorption pits. These findings demonstrate the dose-dependent inhibitory effect of L-quebrachitol on osteoclastogenesis through the modulation of receptor activator of nuclear factor κB (RANK)-mediated signalling pathways. However, further exploration of L-quebrachitol's potential as a therapeutic approach for osteoporosis is warranted.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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