Version 1
: Received: 13 February 2024 / Approved: 13 February 2024 / Online: 13 February 2024 (15:59:01 CET)
How to cite:
Colonna, G. Understanding the SARS-CoV-2-Human Liver Interactome Using a Comprehensive Analysis of the Individual Virus-Host Interactions.. Preprints2024, 2024020757. https://doi.org/10.20944/preprints202402.0757.v1
Colonna, G. Understanding the SARS-CoV-2-Human Liver Interactome Using a Comprehensive Analysis of the Individual Virus-Host Interactions.. Preprints 2024, 2024020757. https://doi.org/10.20944/preprints202402.0757.v1
Colonna, G. Understanding the SARS-CoV-2-Human Liver Interactome Using a Comprehensive Analysis of the Individual Virus-Host Interactions.. Preprints2024, 2024020757. https://doi.org/10.20944/preprints202402.0757.v1
APA Style
Colonna, G. (2024). Understanding the SARS-CoV-2-Human Liver Interactome Using a Comprehensive Analysis of the Individual Virus-Host Interactions.. Preprints. https://doi.org/10.20944/preprints202402.0757.v1
Chicago/Turabian Style
Colonna, G. 2024 "Understanding the SARS-CoV-2-Human Liver Interactome Using a Comprehensive Analysis of the Individual Virus-Host Interactions." Preprints. https://doi.org/10.20944/preprints202402.0757.v1
Abstract
Many metabolic processes at the molecular level support both viral attack strategies and human defenses during Covid-19. This knowledge is of vital importance in the design of antiviral drugs. In this study, we extracted 18 articles (2021-2023) from PubMed reporting the discovery of hub-nodes specific for the liver during covid-19, identifying 142 hub-nodes. They are highly connected proteins from which to get deep functional information on viral strategies when used as functional seeds. Therefore, we evaluated the functional and structural significance of each of them to endorse their reliable use as seeds. After filtering, the remaining 111 hubs were used to get by STRING an enriched interactome of 1111 nodes (13,494 interactions). It shows the viral strategy in the liver is to attack the entire cytoplasmic translational system, including ribosomes, to take control of protein bio-synthesis. We used the SARS2-Human-Proteome Interaction Database (33,791 interactions), de-signed by us with BioGRID data to implement a reverse-engineering process that identified human proteins actively interacting with viral proteins. The results show 57% of human liver proteins di-rectly involved in Covid, a strong impairment of the ribosome and spliceosome, an antiviral defense mechanism against cellular stress of the p53 system, and, surprisingly, a viral capacity for multiple protein attacks against single human proteins that reveal underlying evolutionary-topological molecular mechanisms. Viral behavior over time suggests different molecular strategies for different organs.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
