Version 1
: Received: 3 February 2024 / Approved: 5 February 2024 / Online: 5 February 2024 (15:30:12 CET)
How to cite:
Zhong, J.J.; Zhang, J.; Lin, Y.Y.; Ma, D.J.; Mo, J.; Ye, X.Z. Early Hydrocortisone Therapy Improves Sepsis Outcome in the Neonatal Mice by Inhibiting the NF-κB Pathway: A Pilot Study. Preprints2024, 2024020264. https://doi.org/10.20944/preprints202402.0264.v1
Zhong, J.J.; Zhang, J.; Lin, Y.Y.; Ma, D.J.; Mo, J.; Ye, X.Z. Early Hydrocortisone Therapy Improves Sepsis Outcome in the Neonatal Mice by Inhibiting the NF-κB Pathway: A Pilot Study. Preprints 2024, 2024020264. https://doi.org/10.20944/preprints202402.0264.v1
Zhong, J.J.; Zhang, J.; Lin, Y.Y.; Ma, D.J.; Mo, J.; Ye, X.Z. Early Hydrocortisone Therapy Improves Sepsis Outcome in the Neonatal Mice by Inhibiting the NF-κB Pathway: A Pilot Study. Preprints2024, 2024020264. https://doi.org/10.20944/preprints202402.0264.v1
APA Style
Zhong, J.J., Zhang, J., Lin, Y.Y., Ma, D.J., Mo, J., & Ye, X.Z. (2024). Early Hydrocortisone Therapy Improves Sepsis Outcome in the Neonatal Mice by Inhibiting the NF-κB Pathway: A Pilot Study. Preprints. https://doi.org/10.20944/preprints202402.0264.v1
Chicago/Turabian Style
Zhong, J.J., Jing Mo and Xiu Zhen Ye. 2024 "Early Hydrocortisone Therapy Improves Sepsis Outcome in the Neonatal Mice by Inhibiting the NF-κB Pathway: A Pilot Study" Preprints. https://doi.org/10.20944/preprints202402.0264.v1
Abstract
The unknown timing of appropriate initiation of administration has led to controversy over the use of hydrocortisone in the treatment of neonatal sepsis. This study sought to compare the survival rate and NF-κB pathway expression level in newborn mice treated with hydrocortisone at different initiation times after sepsis to find the appropriate administration time window for clinical neonatal sepsis patients. Before building the sepsis model, 36 newborn C57BL/6 mice were randomly divided into six groups with six mice, using quasi-randomization: five treatment subgroups(1 h, 6 h, 12 h, 24 h and 48 h based on the initiation of hydrocortisone therapy) and 1 control group. The survival rate of mice in each group was compared. The expression levels of the NF-κB pathway and inflammatory factors were analyzed and compared among groups. Initiation of hydrocortisone therapy within 12 h of sepsis onset improved the survival of LPS-induced newborn septic mice. Treatment with hydrocortisone over 12 hours reduced the expression level of NF-κB and lowered serum IL-6 and TNF-α levels in cardiopulmonary tissues of mice. Administration of hydrocortisone early in sepsis (within 12 hours) promotes neonatal mouse survival by inhibiting the NF-κB pathway.
Medicine and Pharmacology, Pediatrics, Perinatology and Child Health
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
