Zenkevich, I.G.; Derouiche, A. Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography. Separations2024, 11, 69.
Zenkevich, I.G.; Derouiche, A. Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography. Separations 2024, 11, 69.
Zenkevich, I.G.; Derouiche, A. Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography. Separations2024, 11, 69.
Zenkevich, I.G.; Derouiche, A. Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography. Separations 2024, 11, 69.
Abstract
Background: Recurrent approximation of retention parameters in reversed-phase HPLC is effective for revealing anomalies difficult to detect otherwise, namely, the reversible hydration of analytes. This was demonstrated previously for restricted sets of analytes with acetonitrile–water eluents. Expanding the number of analytes and eluents seems to be a topical problem. Two kinds of derivatives of aromatic carbonyl compounds were characterized: unsubstituted hydrazones and oximes.
Methods: If analyte demonstrates no anomalies in dependences of retention times vs. concentration of organic modifier, the recurrent approximations of these dependences are linear. To explain the features of recurrent approximations, the numerical experiments were proposed and considered. The artificial shifting of one, two, or more points allows modeling the different kinds of deviations of approximations from linearity.
Results: It was shown that hydrazones are the class of analytes having no anomalies of retention parameters. On the contrary, several anomalies were detected for oximes. Downward deviations of points in the plots of recurrent approximations of retention times are the signs of reversible hydration. This effect for methanol–water eluents was detected for the first time. Some of oximes underwent hydrolysis.
Conclusion: Recurrent approximation of retention times allows detecting chemical transformations of analytes during RP HPLC analysis.
Keywords
Reversed-phase HPLC, retention times, recurrent approximation, detection of anomalies, chemical transformations of analytes.
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
