Version 1
: Received: 1 February 2024 / Approved: 1 February 2024 / Online: 2 February 2024 (06:37:12 CET)
How to cite:
Nazir, T.; A. Mirza, H.; Rao, N. CRISPR-Cas Associated Cells and Animal Mediated Biomedical Modelling. Preprints2024, 2024020027. https://doi.org/10.20944/preprints202402.0027.v1
Nazir, T.; A. Mirza, H.; Rao, N. CRISPR-Cas Associated Cells and Animal Mediated Biomedical Modelling. Preprints 2024, 2024020027. https://doi.org/10.20944/preprints202402.0027.v1
Nazir, T.; A. Mirza, H.; Rao, N. CRISPR-Cas Associated Cells and Animal Mediated Biomedical Modelling. Preprints2024, 2024020027. https://doi.org/10.20944/preprints202402.0027.v1
APA Style
Nazir, T., A. Mirza, H., & Rao, N. (2024). CRISPR-Cas Associated Cells and Animal Mediated Biomedical Modelling. Preprints. https://doi.org/10.20944/preprints202402.0027.v1
Chicago/Turabian Style
Nazir, T., Hameed A. Mirza and Nida Rao. 2024 "CRISPR-Cas Associated Cells and Animal Mediated Biomedical Modelling" Preprints. https://doi.org/10.20944/preprints202402.0027.v1
Abstract
Gene editing is now easy to make new disease models through in-vivo and in vitro tests. It can potentially be used to make animals with single-gene or multiple-gene changes. The mutant strains with changed germlines are no longer needed with in vivo gene editing, which uses the CRISPR-Cas9 system to target cells of interest in their normal tissues. Whereas, the AAVs and other viral vectors have made it possible to change cells selectively. Gene editing hade made it possible to use human induced pluripotent stem cells (iPSC) to model diseases that run in families. Researchers can compare and contrast the human genomes of many different ethnic and racial groups using this method. Scientists may be able to make a disease in a lab dish using iPSCs from a patient. Using CRISPR, iPSCs made from patient cells can be fixed if they have certain problems. This shows that gene therapy is possible and shows what happens when cells aren't working right. The fact that CRISPR-Cas9 can change the DNA by just one nucleotide has had a huge effect on biological studies. CRISPR is becoming more and more popular, which shows how useful, easy, and effective it is. With the broad use of CRISPR-based apps, the tool is now used for much more than just changing genes. This method can be used to screen the whole genome, control the translation of genes based on their sequence, and edit several genes at the same time. Scientists can now model diseases in different species and learn more about how genes work because of these advances. Genome-wide association studies and genome-editing tools like CRISPR are giving us a good look at the future of personalized medicine.
Keywords
CRISPR-Cas System; Genome engineering; Biomedicine; Human disease; Animal models
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
