Technical Note
Version 1
Preserved in Portico This version is not peer-reviewed
Binding versus Catalysis in De Novo Enzyme Design
Version 1
: Received: 30 January 2024 / Approved: 31 January 2024 / Online: 31 January 2024 (11:30:46 CET)
How to cite: Sanchez-Ruiz, J. Binding versus Catalysis in De Novo Enzyme Design . Preprints 2024, 2024012193. https://doi.org/10.20944/preprints202401.2193.v1 Sanchez-Ruiz, J. Binding versus Catalysis in De Novo Enzyme Design . Preprints 2024, 2024012193. https://doi.org/10.20944/preprints202401.2193.v1
Abstract
The catalytic parameters describing enzyme catalysis are discussed in the context of the design of de novo enzymes. It is shown that high values of the catalytic efficiency may in some cases reflect tight substrate binding and lead to an immediate levelling-off of rates to reach a low plateau, an undesirable scenario for many industrial applications. It is argued that increasing the catalytic rate constant should be a main target in de novo enzyme engineering.
Keywords
Protein engineering; de novo enzymes; Michaelis-Menten parameters
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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