Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials

Eva Jablonská; Lucie Mrázková; Jiří Kubásek; Dalibor Vojtěch; Irena Paulin; Tomáš Ruml; Jan Lipov

doi:10.20944/preprints202401.2053.v2

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preprints.org > biology and life sciences > toxicology > doi: 10.20944/preprints202401.2053.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials

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Version 1 : Received: 29 January 2024 / Approved: 30 January 2024 / Online: 30 January 2024 (09:36:25 CET)
Version 2 : Received: 30 January 2024 / Approved: 30 January 2024 / Online: 30 January 2024 (13:56:31 CET)

A peer-reviewed article of this Preprint also exists.

Jablonská, E.; Mrázková, L.; Kubásek, J.; Vojtěch, D.; Paulin, I.; Ruml, T.; Lipov, J. Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials. Materials 2024, 17, 915. Jablonská, E.; Mrázková, L.; Kubásek, J.; Vojtěch, D.; Paulin, I.; Ruml, T.; Lipov, J. Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials. Materials 2024, 17, 915.

Abstract

In vitro testing is the first important step in the development of new biomaterials. The human fetal osteoblast cell line hFOB 1.19 is a very promising cell model; however, there are vast discrepancies in cultivation protocols, especially in the cultivation temperature and the presence of selection reagent, geneticin (G418). We intended to use hFOB 1.19 for the testing of Zn-based degradable metallic materials. However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied. Therefore, we compared the toxicity of zinc towards hFOB 1.19 under different conditions and compared it with that of the L929 mouse fibroblast cell line. We also tested the cytotoxicity of three types of Zn-based biomaterials in two types of media. The presence of G418 used as a selection reagent decreased the sensitivity of hFOB 1.19 to Zn2+. hFOB 1.19 were more sensitive to Zn2+ at elevated (restrictive) temperatures. hFOB 1.19 were less sensitive to Zn2+ than L929 (both as ZnCl2 and extracts of alloys). Therefore, the appropriate cultivation conditions of hFOB 1.19 during biomaterial testing should be chosen with caution.

Keywords

zinc degradable materials, in vitro cytotoxicity testing, hFOB 1.19 osteoblasts

Subject

Biology and Life Sciences, Toxicology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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