Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Titanium-Doped Diamond-Like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells

Martina Travnickova
ORCID logo ,
Elena Filova
* ,
Petr Slepicka
,
Nikola Kasalkova Slepickova
,
Tomas Kocourek
ORCID logo ,
Margit Zaloudkova
,
Tomas Suchy
,
Lucie Bacakova
ORCID logo
Version 1 : Received: 26 January 2024 / Approved: 26 January 2024 / Online: 26 January 2024 (16:42:41 CET)

A peer-reviewed article of this Preprint also exists.

Travnickova, M.; Filova, E.; Slepicka, P.; Slepickova Kasalkova, N.; Kocourek, T.; Zaloudkova, M.; Suchy, T.; Bacakova, L. Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells. Int. J. Mol. Sci. 2024, 25, 2837. Travnickova, M.; Filova, E.; Slepicka, P.; Slepickova Kasalkova, N.; Kocourek, T.; Zaloudkova, M.; Suchy, T.; Bacakova, L. Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells. Int. J. Mol. Sci. 2024, 25, 2837.

Abstract

Diamond-like carbon (DLC) layers are known for their high corrosion and wear resistance, low friction, and high biocompatibility. However, it is often necessary to dope DLC layers with additional chemical elements to strengthen their adhesion to the substrate. Ti-DLC layers (doped with 0.4, 2.1, 3.7, 6.6, 12.8 at.% of Ti) were prepared by dual pulsed laser deposition, and pure DLC, glass, and polystyrene (PS) were used as controls. In vitro cell-material interactions were investigated with an emphasis on cell adhesion, proliferation, and osteogenic differentiation. We observed slightly increasing roughness and contact angle and decreasing surface free energy on Ti-DLC layers with increasing Ti content. Three-week biological experiments were performed using adipose tissue-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (bmM-SCs) in vitro. The cell proliferation activity was similar or slightly higher on the Ti-doped materials than on glass and PS. Osteogenic cell differentiation on all materials was proved by collagen and osteocalcin production, ALP activity, and Ca deposition. The bmMSCs exhibited greater initial proliferation potential and an earlier onset of osteogenic differentiation than the ADSCs. The ADSCs showed a slightly higher formation of focal adhesions, higher metabolic activity, and Ca deposition with increasing Ti content.

Keywords

titanium; diamond-like carbon layer (DLC); biocompatibility; osteogenic differentiation; adipose tissue-derived stem cells (ADSCs); bone marrow mesenchymal stem cells (bmMSCs)

Subject

Medicine and Pharmacology, Orthopedics and Sports Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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