Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals

Aurélien Devillars; Gabriele Magon; Carlotta Pirrello; Fabio Palumbo; Silvia Farinati; Gianni Barcaccia; Margherita Lucchin; Alessandro Vannozzi

doi:10.20944/preprints202401.1774.v1

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preprints.org > biology and life sciences > other > doi: 10.20944/preprints202401.1774.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals

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Version 1 : Received: 24 January 2024 / Approved: 24 January 2024 / Online: 25 January 2024 (01:57:38 CET)

A peer-reviewed article of this Preprint also exists.

Devillars, A.; Magon, G.; Pirrello, C.; Palumbo, F.; Farinati, S.; Barcaccia, G.; Lucchin, M.; Vannozzi, A. Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals. Int. J. Mol. Sci. 2024, 25, 3271. Devillars, A.; Magon, G.; Pirrello, C.; Palumbo, F.; Farinati, S.; Barcaccia, G.; Lucchin, M.; Vannozzi, A. Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals. Int. J. Mol. Sci. 2024, 25, 3271.

Abstract

The advent of CRISPR/Cas9 technology has revolutionized genome editing, enabling the attainment of once-unimaginable goals. CRISPR/Cas's groundbreaking attributes lie in its simplicity, versatility, universality, and its independence from customized DNA-protein systems, erasing the need for specialized expertise and broadening its scope of applications. Beyond its editing prowess, the discovery of novel Cas-based systems has spawned an array of additional biotechnological tools, empowering both fundamental and applied research. Researchers have harnessed CRISPR/Cas for gene regulation, deepening insights into gene expression and epigenetic changes. Precisely targeting DNA or RNA sequences, CRISPR/Cas effectively identifies and counters pathogens. Furthermore, it aids in genome imaging and sequencing, revealing genome spatial organization and chromatin dynamics. The non-editing applications of CRISPR/Cas exhibit tremendous potential across diverse domains, including diagnostics, biotechnology, and fundamental research. This article reviews and critically evaluates the primary CRISPR/Cas-based tools developed for plants and animals, underlining their transformative impact.

Keywords

CRISPR/Cas; Imaging; Gene regulation; NGS; Viruses; CRISPR/Cas9; CRISPR/Cas13; dCas9

Subject

Biology and Life Sciences, Other

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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