Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Two Novel Betarhabdovirids Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii

Pedro Luis Ramos-González
* ORCID logo ,
Maria Amelia Vaz Alexandre
,
Matheus Potsclam-Barro
,
Lígia Maria Lembo Duarte
ORCID logo ,
Gianluca L. Michea Gonzalez
,
Camila Chabi-Jesus
ORCID logo ,
Alyne F. Ramos
,
Ricardo Harakava
,
Harri Lorenzi
,
Juliana Freitas-Astúa
ORCID logo ,
Elliot Watanabe Kitajima
Version 1 : Received: 19 January 2024 / Approved: 23 January 2024 / Online: 23 January 2024 (09:50:15 CET)

A peer-reviewed article of this Preprint also exists.

Ramos-González, P.L.; Alexandre, M.A.V.; Potsclam-Barro, M.; Duarte, L.M.L.; Michea Gonzalez, G.L.; Chabi-Jesus, C.; Ramos, A.F.; Harakava, R.; Lorenzi, H.; Freitas-Astúa, J.; Kitajima, E.W. Two Novel Betarhabdovirins Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii. Viruses 2024, 16, 322. Ramos-González, P.L.; Alexandre, M.A.V.; Potsclam-Barro, M.; Duarte, L.M.L.; Michea Gonzalez, G.L.; Chabi-Jesus, C.; Ramos, A.F.; Harakava, R.; Lorenzi, H.; Freitas-Astúa, J.; Kitajima, E.W. Two Novel Betarhabdovirins Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii. Viruses 2024, 16, 322.

Abstract

Two novel members of the subfamily Betarhabdovirinae, family Rhabdoviridae, were identified in Brazil. Overall, their genomes have the typical organization 3’-N-P-P3-M-G-L-5’ observed in mono-segmented plant-infecting rhabdoviruses. In aristolochia-associated cytorhabdovirus (AaCV), found in the liana aristolochia (Aristolochia gibertii Hook), an additional short orphan ORF encoding a transmembrane helix was detected between P3 and M. AaCV genome and deduced encoded proteins share the highest identity values, consistently < 60%, with their counterparts of yerba mate chlorosis-associated virus (Cytorhabdovirus flaviyerbamate). The second virus, false jalap virus (FaJV), was detected in the herbaceous plant false jalap (Mirabilis jalapa L.) and represents together with tomato betanucleorhabdovirus 2, originally found in tomato plants in Slovenia, a tentative new species of the genus Betanucleorhabdovirus. FaJV particles accumulate in the peri-nuclear space, and electron-lucent viroplasms were observed in the nuclei of the infected cells. Notably, distinct from typical rhabdoviruses, most virions of AaCV were observed to be non-enclosed within membrane-bounded cavities. Instead, they were frequently seen in close association with surfaces of mitochondria or peroxisomes. Unlike FaJV, AaCV was successfully graft-transmitted to healthy plants of three species of the genus Aristolochia, while mechanical and seed transmission proved unsuccessful for both viruses. Data suggest that these viruses belong to two new tentative species within the subfamily Betarhabdovirinae.

Keywords

rhabdovirus; betanucleorhabdovirus; high-throughput sequencing; virion particles; cytopathology

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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