Wang, Q.; Zhao, J.; Liu, Z.; Ding, S.; Huang, Z.; Chen, J. Genomic Insights and Synthetic Biology Applications of Marine Actinomycete Streptomyces griseoincarnatus HNS054. Int. J. Mol. Sci.2024, 25, 3127.
Wang, Q.; Zhao, J.; Liu, Z.; Ding, S.; Huang, Z.; Chen, J. Genomic Insights and Synthetic Biology Applications of Marine Actinomycete Streptomyces griseoincarnatus HNS054. Int. J. Mol. Sci. 2024, 25, 3127.
Wang, Q.; Zhao, J.; Liu, Z.; Ding, S.; Huang, Z.; Chen, J. Genomic Insights and Synthetic Biology Applications of Marine Actinomycete Streptomyces griseoincarnatus HNS054. Int. J. Mol. Sci.2024, 25, 3127.
Wang, Q.; Zhao, J.; Liu, Z.; Ding, S.; Huang, Z.; Chen, J. Genomic Insights and Synthetic Biology Applications of Marine Actinomycete Streptomyces griseoincarnatus HNS054. Int. J. Mol. Sci. 2024, 25, 3127.
Abstract
Streptomyces sp. HNS054, which was previously isolated from a marine sponge, demonstrates vigorous growth and facile genetic modification. This study explores its potential as a marine ac-tinomycete chassis through complete genome sequencing and genetic engineering. With a 7.5 Mb genome containing 6678 predicted genes, 21 secondary metabolic biosynthetic gene clusters (BGCs), and 8 common site-specific recombination (SSR) system attB loci, HNS054 shows a close phylogenetic relation to S. griseoincarnatus strain RB7AG. Utilizing multiplexed site-specific ge-nome engineering (MSGE) and the CRISPR/Cas9 method, engineered strains derived from HNS054 (with three copies of the target BGCs) produce higher amounts of aborycin and ac-tinorhodin than S. coelicolor M1346 (containing three copies of the same BGCs). HNS054 stands out for its remarkable characteristics, including salt tolerance, rapid growth, and compatibility with synthetic biology tools, and positions it as a promising candidate for developing marine ac-tinomycete hosts to enhance secondary metabolite production.
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