Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Human Papillomavirus E6 /E7 mRNA Testing in the Follow-Up of Patients Treated for High-Grade Cervical Intraepithelial Neoplasia

Adolfo Loayza Galindo
* ORCID logo ,
Alicia Hernandez Gutierrez
,
Ana Margarita Rodriguez Garcia
,
Belen Lopez Cavanillas
,
Cristina Gonzalez Benitez
,
David Hardisson
ORCID logo ,
Itziar De la Peña Navarro
,
Maria Serrano Velasco
,
Rocio Arnedo Muñoz
,
Ignacio Zapardiel
ORCID logo
Version 1 : Received: 21 January 2024 / Approved: 22 January 2024 / Online: 22 January 2024 (09:03:33 CET)

How to cite: Loayza Galindo, A.; Hernandez Gutierrez, A.; Rodriguez Garcia, A. M.; Lopez Cavanillas, B.; Gonzalez Benitez, C.; Hardisson, D.; De la Peña Navarro, I.; Velasco, M. S.; Arnedo Muñoz, R.; Zapardiel, I. Human Papillomavirus E6 /E7 mRNA Testing in the Follow-Up of Patients Treated for High-Grade Cervical Intraepithelial Neoplasia. Preprints 2024, 2024011576. https://doi.org/10.20944/preprints202401.1576.v1 Loayza Galindo, A.; Hernandez Gutierrez, A.; Rodriguez Garcia, A. M.; Lopez Cavanillas, B.; Gonzalez Benitez, C.; Hardisson, D.; De la Peña Navarro, I.; Velasco, M. S.; Arnedo Muñoz, R.; Zapardiel, I. Human Papillomavirus E6 /E7 mRNA Testing in the Follow-Up of Patients Treated for High-Grade Cervical Intraepithelial Neoplasia. Preprints 2024, 2024011576. https://doi.org/10.20944/preprints202401.1576.v1

Abstract

In the follow-up of treated high-grade cervical intraepithelial neoplasia (HSIL/CIN) lesions, cer-vical cytology has a high false-negative rate, while high-risk human papillomavirus (HR-HPV) DNA testing showed high sensitivity and lower specificity. The detection of messenger RNA of the HR-HPV E6 and E7 oncoproteins (E6/E7 mRNA) seems to be an indicator of viral integration and it allows the identification of severe lesions. Our objective was to assess the clinical utility of E6/E7 mRNA determination in the follow-up of patients treated for HSIL/CIN. A retrospective obser-vational study including 407 patients treated for HSIL/CIN was performed. The recurrence rate and the validity of E6/E7 mRNA testing were evaluated. The recurrence rate of high-grade lesions was 1.7%. The sensitivity of the test was 88% in the first clinical revision and 100% in the second and third revisions. Specificity was 91% in the first revision, 92% in the second revision, and 85% in the third revision. The positive predictive value was 18% in the first medical revision, 10% in the second and 14% in the third. The negative predictive value was 100% in all follow-up visits. In conclusion, the E6/E7 mRNA test seems to be effective in ruling out recurrence after treatment for HSIL/CIN lesions.

Keywords

cervical intraepithelial neoplasia; conization; human papillomavirus; E6 and E7 mRNA.

Subject

Public Health and Healthcare, Public Health and Health Services

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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