Oishi, T.; Hattori, N.; Yoshioka, D. Novel Knowledge of Macrolide Resistance in Mycoplasma pneumoniae by Azithromycin Exposure. Microorganisms2024, 12, 218.
Oishi, T.; Hattori, N.; Yoshioka, D. Novel Knowledge of Macrolide Resistance in Mycoplasma pneumoniae by Azithromycin Exposure. Microorganisms 2024, 12, 218.
Oishi, T.; Hattori, N.; Yoshioka, D. Novel Knowledge of Macrolide Resistance in Mycoplasma pneumoniae by Azithromycin Exposure. Microorganisms2024, 12, 218.
Oishi, T.; Hattori, N.; Yoshioka, D. Novel Knowledge of Macrolide Resistance in Mycoplasma pneumoniae by Azithromycin Exposure. Microorganisms 2024, 12, 218.
Abstract
The rise of macrolide-resistant Mycoplasma pneumoniae (MRMP), marked bypoint mutations in the 23S rRNA gene, poses a growing global concern since its initial detection in 2001. The prominence of the A2063G mutation during this emergence remains unexplained. This study aimed to clarify the possibility of detecting MRMP from recent clinical macrolide-susceptible M. pneumoniae by exposure to azithromycin (AZM), which has a long half-life and was launched immediately before the first MRMP detection. Six strains isolated from Japanese children in 2019 and reference strain (FH), all belonging to the recent dominant P1 genotype, two or two subtype, were cultivated in a medium containing slightly higher concentrations than the originated minimum inhibitory concentration (MIC) of AZM and underwent sequencing if they grew. Four out of the seven strains grew after exposure to AZM, and C2617G, C2617A were detected, with no mutation in two strains. After another cultivation and sequencing, two of four strains grew and one was changed from C2617G to A2063G, and the other remained C2617A. The MIC of AZM in A2063G strains was 128 mg/mL; for C2617A, it was 0.0156 mg/mL. This is the first study to detect the strains with A2063G mutation from recent macrolide-susceptible M. pneumoniae using AZM exposure.
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