Version 1
: Received: 16 January 2024 / Approved: 17 January 2024 / Online: 17 January 2024 (04:22:38 CET)
Version 2
: Received: 19 February 2024 / Approved: 20 February 2024 / Online: 20 February 2024 (06:30:15 CET)
How to cite:
Zhao, X.; Bhat, A.; O’Connor, C.; Curtin, J.; Singh, B.; Tian, F. Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective. Preprints2024, 2024011268. https://doi.org/10.20944/preprints202401.1268.v2
Zhao, X.; Bhat, A.; O’Connor, C.; Curtin, J.; Singh, B.; Tian, F. Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective. Preprints 2024, 2024011268. https://doi.org/10.20944/preprints202401.1268.v2
Zhao, X.; Bhat, A.; O’Connor, C.; Curtin, J.; Singh, B.; Tian, F. Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective. Preprints2024, 2024011268. https://doi.org/10.20944/preprints202401.1268.v2
APA Style
Zhao, X., Bhat, A., O’Connor, C., Curtin, J., Singh, B., & Tian, F. (2024). Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective. Preprints. https://doi.org/10.20944/preprints202401.1268.v2
Chicago/Turabian Style
Zhao, X., Baljit Singh and Furong Tian. 2024 "Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective" Preprints. https://doi.org/10.20944/preprints202401.1268.v2
Abstract
Foodborne illnesses can be infectious and dangerous and most of them are caused by bacteria. Some types of common food-related bacteria exist widely in nature and pose a serious threat to both humans and animals, and can cause poisoning, diseases, disabilities and even death. Rapid, reliable and cost-effective methods for bacteria detection are of paramount importance in food-safety and environmental monitoring. Polymerase chain reaction (PCR), lateral flow immunochromatographic assay (LFIA) and electrochemical method have been widely used in food-safety and environmental monitoring. In this paper, the recent developments (2013-2023) covering PCR, LFIA and electrochemical method for various bacteria detection (Salmonella, Listeria, Campylobacter, Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)) considering different foods types, analytical performances and the reported limit of detection (LOD) are discussed. It is found that the bacteria type and food sample type contribute significantly to the analytical performance and LOD. Detection by LFIA has higher average LOD (24 CFU/ml) than detection by electrochemical method (12 CFU/ml) and PCR (6 CFU/ml). Salmonella and E. coli in the Pseudomonadota domain usually have low LODs. LODs are usually lower for detection in fish and eggs. LFIA with gold and iron nanoparticles are prominent in the majority of articles of 26 CFU/ml and 12 CFU/ml respectively. Electrochemical method reveals that the average LOD is highest for cyclic voltammetry (CV) of 18 CFU/ml), followed by electrochemical impedance spectroscopy (EIS) of 12 CFU/ml and differential pulse voltammetry (DPV) of 8 CFU/ml. LOD usually decreases when replicate number increases until it remain unchanged. Exponential relations with (R2>0.95) between LOD of Listeria in milk by LFIA and electrochemical method with replicate number have been obtained. Finally, the review discusses the challenges and future perspectives (including the role of nanomaterials/advanced materials) to improve the analytical performance for bacterial detection.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
