Version 1
: Received: 15 January 2024 / Approved: 16 January 2024 / Online: 16 January 2024 (08:22:35 CET)
How to cite:
Li, Z.; Liao, Y.; Liu, M.; Qin, L.; Wang, J.; Zu, Y. Pb Accumulation Related to Plant Hormones and Its Mechanism Based on Genes Expression and Transporter Activities in Hyperaccumulator Arabis alpina. Preprints2024, 2024011180. https://doi.org/10.20944/preprints202401.1180.v1
Li, Z.; Liao, Y.; Liu, M.; Qin, L.; Wang, J.; Zu, Y. Pb Accumulation Related to Plant Hormones and Its Mechanism Based on Genes Expression and Transporter Activities in Hyperaccumulator Arabis alpina. Preprints 2024, 2024011180. https://doi.org/10.20944/preprints202401.1180.v1
Li, Z.; Liao, Y.; Liu, M.; Qin, L.; Wang, J.; Zu, Y. Pb Accumulation Related to Plant Hormones and Its Mechanism Based on Genes Expression and Transporter Activities in Hyperaccumulator Arabis alpina. Preprints2024, 2024011180. https://doi.org/10.20944/preprints202401.1180.v1
APA Style
Li, Z., Liao, Y., Liu, M., Qin, L., Wang, J., & Zu, Y. (2024). Pb Accumulation Related to Plant Hormones and Its Mechanism Based on Genes Expression and Transporter Activities in Hyperaccumulator <em>Arabis alpina</em>. Preprints. https://doi.org/10.20944/preprints202401.1180.v1
Chicago/Turabian Style
Li, Z., Jixiu Wang and Yanqun Zu. 2024 "Pb Accumulation Related to Plant Hormones and Its Mechanism Based on Genes Expression and Transporter Activities in Hyperaccumulator <em>Arabis alpina</em>" Preprints. https://doi.org/10.20944/preprints202401.1180.v1
Abstract
Plants endogenous hormones play an important role in resistance to soil lead (Pb) contamination. In order to explore the response of plant hormones to Pb stress, pot experiment was conducted to analysis the hormones contents, related gene expressions and Pb accumulation of Arabis alpina under Pb treatment and transporter (CAX(cation exchangers), HMA(heavy metal ATPase) and ABC(ATP-binding cassette transporter)) activities under foliage spraying auxin (IAA). The results showed that contents of total Pb and soluble components Pb (vacuoles) in roots and leaves of Arabis alpina increased with increase of Pb treatment concentrations. Compering to 100 mg·kg-1 Pb2+ treatment, Pb contents in soluble components of roots and leaves under 300 mg·kg-1 Pb2+ treatment increased by 896.3% and 238.8%, respectively. The contents of endogenous hormones in leaves and roots increased under Pb treatment concentrations. Comparing to control (0 mg·kg-1 Pb2+ treatment), contents of auxin in roots and leaves under 100 mg∙kg-1 Pb treatment increased by 176.19% and 585.29%, respectively. Auxin contents in xylem saps under 100 and 300 mg∙kg-1 Pb treatments increased by 283.14% and 100.30%, respectively. Gene expression related to auxin transport was up-adjusted. The expressions of three gene related to auxin-repressed 12.5 kDa protein and auxin-responsive GH3 family were down-adjusted. Under foliage spraying IAA, Pb contents in leaves increased by 29.81% and Pb content in symplast sap was higher than that with non spraying IAA treatment. The activities of CAX and HMA in roots of A. alpina increased by 9.62% and 8.79% with foliage spraying IAA treatment, while activity of ABC decreased by 21.94%. In general, contents of auxin and related genes expressions of A. alpina increased under Pb treatment, which enhanced activities of CAX and HMA resulting in Pb translocation through symplast pathway.
Environmental and Earth Sciences, Environmental Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
