Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Anti-cariogenic Effects of S. cerevisiae and S. boulardii in S. mutans–C. albicans Cross-Kingdom In Vitro Model

Dina Yousif
,
Yan Wu
ORCID logo ,
Alexandria Azul Gonzales
ORCID logo ,
Christa Mathieu
,
Yan Zeng
,
Sample Lee
,
Sabrina Terando
,
Ting Li
,
Jin Xiao
*
Version 1 : Received: 11 January 2024 / Approved: 11 January 2024 / Online: 11 January 2024 (08:04:48 CET)

A peer-reviewed article of this Preprint also exists.

Yousif, D.; Wu, Y.; Gonzales, A.A.; Mathieu, C.; Zeng, Y.; Sample, L.; Terando, S.; Li, T.; Xiao, J. Anti-Cariogenic Effects of S. cerevisiae and S. boulardii in S. mutans–C. albicans Cross-Kingdom In Vitro Models. Pharmaceutics 2024, 16, 215. Yousif, D.; Wu, Y.; Gonzales, A.A.; Mathieu, C.; Zeng, Y.; Sample, L.; Terando, S.; Li, T.; Xiao, J. Anti-Cariogenic Effects of S. cerevisiae and S. boulardii in S. mutans–C. albicans Cross-Kingdom In Vitro Models. Pharmaceutics 2024, 16, 215.

Abstract

Despite well-documented health benefits of probiotics Saccharomyces, its application in oral health has not been comprehensively assessed. Dental caries is a transmissible disease initiated by acid production of cariogenic bacteria and yeast, such as Streptococcus mutans and Candida albicans, on tooth enamel and followed by subsequent enamel demineralization. Here, we investigated the effect of two Saccharomyces strains (Saccharomyces boulardii, Saccharomyces cerevisiae) on S. mutans-C. albicans cross-kingdom interactions using a cariogenic planktonic model. Viable cells, pH changes and gene expression were measured. S. cerevisiae and S. boulardii inhibited the growth of C. albicans either in dual-, or multi-species conditions. Saccharomyces also inhibited C. albicans hyphal formation. Furthermore, Saccharomyces reduced the acidity of the culture medium which usually plummeted below pH 5 when S. mutans and C. albicans were present in the model. The presence of Saccharomyces maintained the culture medium above 6 even after overnight incubation, demonstrating a protective potential against dental enamel demineralization. S. boulardii significantly downregulated S. mutans atpD and eno genes expression. Overall, our results shed light on a new promising candidate, Saccharomyces, for dental caries prevention due to its potential to create a less cariogenic environment marked by a neutral pH and reduced growth of C. albicans.

Keywords

Saccharomyces cerevisiae; Saccharomyces boulardii; Candida albicans; cross-kingdom interaction; pH; Dental caries

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.