Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In Vitro Gastrointestinal Digestion of Dietary Fiber Rich Ruzeiz Date Pomace: Bioaccessibility and Stability of the Phenolic Compounds and Antioxidants.

Version 1 : Received: 8 January 2024 / Approved: 10 January 2024 / Online: 11 January 2024 (02:57:59 CET)

How to cite: Alhuthayli, H.F.; Mohammed Ahmed, M.A.A.; Zarie, A.A.; Al kharashi, N.A.; Aljasass, F.M.; Alkhudhayri, D.A.; Osman, M.A. In Vitro Gastrointestinal Digestion of Dietary Fiber Rich Ruzeiz Date Pomace: Bioaccessibility and Stability of the Phenolic Compounds and Antioxidants.. Preprints 2024, 2024010825. https://doi.org/10.20944/preprints202401.0825.v1 Alhuthayli, H.F.; Mohammed Ahmed, M.A.A.; Zarie, A.A.; Al kharashi, N.A.; Aljasass, F.M.; Alkhudhayri, D.A.; Osman, M.A. In Vitro Gastrointestinal Digestion of Dietary Fiber Rich Ruzeiz Date Pomace: Bioaccessibility and Stability of the Phenolic Compounds and Antioxidants.. Preprints 2024, 2024010825. https://doi.org/10.20944/preprints202401.0825.v1

Abstract

This study investigated the chemical composition, and total phenolic content(TPC), total flavonoid content (TFC), and antioxidant activity Reziz date pomace extracted using different solvent system. The recovery, bioaccessibility, and stability of bioactive compounds were evaluated using In vitro gastrointestinal digestion. The results showed that pomace rich source of fiber but low in fat, and protein. Extraction data revealed that methanol, and ethanol were the most effective solvent for TPC and TFC extraction with higher antioxidant activity. In vitro gastrointestinal digestibility significantly affected recovery, bio-accessibility and stability of bioactive compounds. The TPC, TFC, and antioxidant activities were significantly increased after the mouth digestion but decreased during the gastric and intestinal digestion. The bioaccessibility of polyphenol and flavonoid were 35.22% and 23.57% respectively after the intestinal digestion. The chyme insoluble fraction (pellet) showed higher bioactive compounds and antioxidant activity compared to the chyme soluble fraction. The high fiber content of the date pomace seemed to affect the bioaccessibility of the bioactive compounds. TPC or TFC and the antioxidant activity determined by DPPH and ABTS were found to have a positive correlation with the oral, gastric, and intestinal digestion. The GC-MS analysis of digested date pomace in mouth, gastric, or intestinal digestion revealed an increase in the number of peaks (compounds) compared to the undigested sample. According to these findings, Ruzeiz date pomace could be utilized as a bioactive ingredient source in formulation of functional foods to promote healthy life.

Keywords

Date Pomace; Phenolic compounds; antioxidants activities; gastrointestinal in-vitro digestibility; Recovery; bioaccessibility; stability

Subject

Public Health and Healthcare, Other

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