preprints.org > engineering > bioengineering > doi: 10.20944/preprints202401.0617.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 5 January 2024 / Approved: 5 January 2024 / Online: 8 January 2024 (16:22:14 CET)

3D bioprinting, like 3D printing, is a process that builds structures by depositing material layer by layer. In the specific case of bioprinting, these layers are composed of a biocompatible material together with living cells, allowing the creation of three-dimensional structures that recreate functional tissues and organs. Bioprinting cell-laden structures is complicated by the high rate of cell damage and stress that occurs during the process, caused by the high pressures and stresses to which they are subjected. To minimise damage during the process, it is important to study and optimise certain bioprinting conditions beforehand and to analyse how they affect the cells by means of computational simulations. In this review, both quantitative and qualitative data are collected to improve the nozzle geometry through computational simulation studies. Optimal ranges for nozzle diameter (0.2-1mm) and length (8-10mm; 300-900μm) have been defined, and recommendations for improving nozzle performance during bioprinting, such as the provision of an inner angle of 20-30°, an inner EDTA coating and a shear stress of less than 10kPa, have been gathered. Finally, based on the data collected, a design of experiments is proposed to obtain an optimal bioprinting configuration for a particular bioink.

bioprinting; microextrusion; computational simulation; nozzle

Engineering, Bioengineering

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