PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Repeated Superstimulation Facilitated Ovum Pick-Up and In Vitro Production (OPU-IVP) of Viable Bovine Embryos with No Apparent Adverse Effects on Donor Health
Version 1
: Received: 3 January 2024 / Approved: 4 January 2024 / Online: 4 January 2024 (15:17:50 CET)
How to cite:
Guest, A.; Salek, F.; Johnson, C.; Kastelic, J.P.; Thundathil, J. Repeated Superstimulation Facilitated Ovum Pick-Up and In Vitro Production (OPU-IVP) of Viable Bovine Embryos with No Apparent Adverse Effects on Donor Health. Preprints2024, 2024010308. https://doi.org/10.20944/preprints202401.0308.v1
Guest, A.; Salek, F.; Johnson, C.; Kastelic, J.P.; Thundathil, J. Repeated Superstimulation Facilitated Ovum Pick-Up and In Vitro Production (OPU-IVP) of Viable Bovine Embryos with No Apparent Adverse Effects on Donor Health. Preprints 2024, 2024010308. https://doi.org/10.20944/preprints202401.0308.v1
Guest, A.; Salek, F.; Johnson, C.; Kastelic, J.P.; Thundathil, J. Repeated Superstimulation Facilitated Ovum Pick-Up and In Vitro Production (OPU-IVP) of Viable Bovine Embryos with No Apparent Adverse Effects on Donor Health. Preprints2024, 2024010308. https://doi.org/10.20944/preprints202401.0308.v1
APA Style
Guest, A., Salek, F., Johnson, C., Kastelic, J.P., & Thundathil, J. (2024). Repeated Superstimulation Facilitated Ovum Pick-Up and In Vitro Production (OPU-IVP) of Viable Bovine Embryos with No Apparent Adverse Effects on Donor Health. Preprints. https://doi.org/10.20944/preprints202401.0308.v1
Chicago/Turabian Style
Guest, A., John Patrick Kastelic and Jacob Thundathil. 2024 "Repeated Superstimulation Facilitated Ovum Pick-Up and In Vitro Production (OPU-IVP) of Viable Bovine Embryos with No Apparent Adverse Effects on Donor Health" Preprints. https://doi.org/10.20944/preprints202401.0308.v1
Abstract
Objectives of this study were to investigate effects of repeated superstimulation on the success of ovum-pick up and in vitro production of embryos (OPU-IVP) and donor health and evaluate the efficiency of a modified SOF sequential medium in producing viable embryos. Over 1 y, six cross-bred beef heifers and three beef cows were subjected to superstimulations and OPU-IVP. Oocytes recovered were used for in vitro embryo production and the resulting embryos were transferred as fresh or frozen-thawed. Number of follicles, oocytes recovered, cleavage and blastocyst rates substantially varied among individuals. However, repeated trials did not significantly affect the efficiency of OPU-IVP. The pregnancy rates (PR) 22 to 30 d after embryo transfer for in vitro-derived fresh embryos was similar to that of fresh in vivo embryos, whereas transfer of in vitro-derived frozen embryos reduced PR compared to frozen-thawed in vivo embryos. All recipient heifers diagnosed pregnant delivered a viable calf, suggesting developmental competence of the embryos. Repeated OPU-IVP had no apparent deleterious effects, as all donors had viable calves after ET. We concluded that multiple OPU-IVP cycles can be effectively performed for a prolonged interval to maximize embryo production from elite donors without compromising their reproductive health.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
