Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Immunological Response against Breast Lineage Cells Transfected with Human Papillomavirus (HPV)

Daffany Luana dos Santos
,
Bianca de França São Marcos
,
Georon Ferreira De Sousa
ORCID logo ,
Leonardo Carvalho de Oliveira Cruz
,
Bárbara Rafaela da Silva Barros
,
Mariane Cajuba de Britto Lira Nogueira
,
Talita Helena de Araújo Oliveira
,
Anna Jéssica Duarte Silva
ORCID logo ,
Vanessa Emanuelle Pereira Santos
,
Cristiane Moutinho Lagos De Melo
,
Antonio Carlos De Freitas
* ORCID logo
Version 1 : Received: 18 December 2023 / Approved: 4 January 2024 / Online: 4 January 2024 (03:06:53 CET)

How to cite: Santos, D.L.D.; São Marcos, B.D.F.; De Sousa, G.F.; Cruz, L.C.D.O.; Barros, B.R.D.S.; Nogueira, M.C.D.B.L.; Oliveira, T.H.D.A.; Silva, A.J.D.; Santos, V.E.P.; De Melo, C.M.L.; De Freitas, A.C. Immunological Response against Breast Lineage Cells Transfected with Human Papillomavirus (HPV). Preprints 2024, 2024010301. https://doi.org/10.20944/preprints202401.0301.v1 Santos, D.L.D.; São Marcos, B.D.F.; De Sousa, G.F.; Cruz, L.C.D.O.; Barros, B.R.D.S.; Nogueira, M.C.D.B.L.; Oliveira, T.H.D.A.; Silva, A.J.D.; Santos, V.E.P.; De Melo, C.M.L.; De Freitas, A.C. Immunological Response against Breast Lineage Cells Transfected with Human Papillomavirus (HPV). Preprints 2024, 2024010301. https://doi.org/10.20944/preprints202401.0301.v1

Abstract

Breast cancer represents the most common neoplasm worldwide. Viral infections are involved with carcinogenesis, especially those caused by oncogenic Human Papillomavirus (HPV) genotypes. The relationship between HPV and various types of cancer, such as breast and lung, still requires further studies. After infection with HPV, there is a remodeling of the tumor microenvironment, providing a post-infection alteration with immunosuppressive characteristics. This study evaluated the role of HPV in breast adenocarcinoma cell lineage (MDA-MB-231), transfected with the HPV16 E5, E6, and E7 oncogenes and co-cultured with PBMCs. Immunophenotyping was performed. There was an increase in CD4+ T cells number when compared with non-transfected and transfected MDA-MB-231, especially the Treg profile. Moreover, proinflammatory intracellular cytokines, such as IFN-γ, TNF-α, and IL-17 were impaired by transfected cells and a decrease in the cytolytic ac-tivity of the CD8+ and CD56+ lymphocytes was also observed in the presence of HPV oncogenes, mainly with E6 and E7 in NK and TCD8+ cells. In conclusion, MDA-MB-231 breast cell lineage transfected with HPV oncogenes can downregulate the number and function of lymphocytes and monocytes.

Keywords

Keywords: oncogenes; MDA-MB-231; triple-negative.

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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