Version 1
: Received: 2 January 2024 / Approved: 3 January 2024 / Online: 3 January 2024 (11:59:25 CET)
Version 2
: Received: 4 January 2024 / Approved: 4 January 2024 / Online: 5 January 2024 (12:06:10 CET)
Sugimori, T.; Yamaguchi, M.; Kikuta, J.; Shimizu, M.; Negishi, S. Micro-Osteoperforations Accelerate Tooth Movement without Exacerbating the Progression of Root Resorption in Rats. Biomolecules2024, 14, 300.
Sugimori, T.; Yamaguchi, M.; Kikuta, J.; Shimizu, M.; Negishi, S. Micro-Osteoperforations Accelerate Tooth Movement without Exacerbating the Progression of Root Resorption in Rats. Biomolecules 2024, 14, 300.
Sugimori, T.; Yamaguchi, M.; Kikuta, J.; Shimizu, M.; Negishi, S. Micro-Osteoperforations Accelerate Tooth Movement without Exacerbating the Progression of Root Resorption in Rats. Biomolecules2024, 14, 300.
Sugimori, T.; Yamaguchi, M.; Kikuta, J.; Shimizu, M.; Negishi, S. Micro-Osteoperforations Accelerate Tooth Movement without Exacerbating the Progression of Root Resorption in Rats. Biomolecules 2024, 14, 300.
Abstract
A recent study reported that micro-osteoperforations (MOPs) accelerated tooth movement by activating alveolar bone remodeling. However, very little is known about the relationship between MOPs and external apical root resorption during orthodontic treatment. In this study, in order to investigate the mechanism which MOPs accelerate tooth movement without exacerbating the progression of root resorption, we measured the volume of resorbed root, and performed the immunostaining for the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end-labelling method (TUNEL) exposed MOPs during rat experimental tooth movement. Male Wistar rats (11 week old) were divided into 3 groups: 10-g orthodontic force (optimal force) applied to the maxillary first molar (optimal force: OF group), 50-g orthodontic force application (heavy force: HF group) and 10-g force application plus 3 small perforations of the cortical plate (OF+MOPs group). On days 1, 4, 7, 10 and 14 after force application, the tooth movement and root volume were investigated by micro-computed tomography. Furthermore, the expression levels of apoptotic cells in pressure sides of periodontal ligament (PDL) and surround hard tissues were determined by TUNEL staining. The OF+MOP group exhibited a 1.8-fold increase in tooth movement on days 7, 10 and 14 compared with the OF group. On days 14, the HF group had a higher volume of root loss than the OF and OF+MOP groups. On the same day, the number of TUNEL-positive cells in the HF group increased at the root (cementum) site whereas that in the OF group increased at the alveolar bone site. Furthermore, that in the OF + MOP group increased at the alveolar bone site compared with the OF group. These results suggest that MOPs accelerate orthodontic tooth movement without exacerbating the progression of root resorption.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter: Masaru Yamaguchi
Commenter's Conflict of Interests: Author