Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Bacteriophage St_134 and its Endolysin as a Potential Staphylococcus Biofilm-Removing Biological Agent

Natalia N. Golosova
,
Andrey L. Matveev
* ORCID logo ,
Nina V. Tikunova
,
Yana A. Khlusevich
ORCID logo ,
Yulia N. Kozlova
,
Vera V. Morozova
ORCID logo ,
Igor V. Babkin
,
Tatiana A. Ushakova
,
Elena V. Zhirakovskaya
ORCID logo ,
Elisaveta A. Panina
,
Elena I. Ryabchikova
,
Artem Y. Tikunov
* ORCID logo
Version 1 : Received: 21 December 2023 / Approved: 21 December 2023 / Online: 21 December 2023 (11:33:13 CET)
Version 2 : Received: 22 January 2024 / Approved: 22 January 2024 / Online: 23 January 2024 (00:07:48 CET)

A peer-reviewed article of this Preprint also exists.

Golosova, N.N.; Matveev, A.L.; Tikunova, N.V.; Khlusevich, Y.A.; Kozlova, Y.N.; Morozova, V.V.; Babkin, I.V.; Ushakova, T.A.; Zhirakovskaya, E.V.; Panina, E.A.; Ryabchikova, E.I.; Tikunov, A.Y. Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents. Viruses 2024, 16, 385. Golosova, N.N.; Matveev, A.L.; Tikunova, N.V.; Khlusevich, Y.A.; Kozlova, Y.N.; Morozova, V.V.; Babkin, I.V.; Ushakova, T.A.; Zhirakovskaya, E.V.; Panina, E.A.; Ryabchikova, E.I.; Tikunov, A.Y. Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents. Viruses 2024, 16, 385.

Abstract

Bacteria of the genus Staphylococcus are a significant a challenge for medicine, as many species are resistant to multiple antibiotics and even to all of the antibiotics used. One of the approaches to develop new therapeutics to treat staphylococcal infections is the use of bacteriophages specif-ic to these bacteria or lysins of such bacteriophages capable to hydrolyze the cell walls of these bacteria. In this study, a new bacteriophage St_134 specific to Staphylococcus epedermidis was de-scribed. This small podophage belonging to the Andhravirus genus showed relatively wide host range and was able to infect more than 60 strain of Staphylococcus spp., including a clinical strain from the Staphylococcus aureus complex. Two genes encoding different phage lysins were identi-fied in the Ste134 genome. Both lysins LysSte134_1 and LysSte134_2 were produced in Escherichia coli cells. Endolysin LysSte134_1 was demonstrated catalytic activity against a wide panel of staphylococcal peptidoglycans (4 species, 6 strains), was active against S. aureus and S. epidermidis planktonic cultures and destroyed biofilms formed by clinical strains of S. aureus and S. epider-midis.

Keywords

antibiotic resistance; bacteriophage; endolysin; antimicrobial agent; Staphylococcus aureus; Staphylococcus epidermidis; biofilm

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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