Detection of Parasites in the Field: The Ever-Innovating CRISPR-Cas12a

Xin LI; Zhisheng DANG; Wenqiang TANG; Haoji ZHANG; Jianwei SHAO; Rui JIANG; Xu ZHANG; Fuqiang HUANG

doi:10.20944/preprints202312.1636.v1

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preprints.org > biology and life sciences > parasitology > doi: 10.20944/preprints202312.1636.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Detection of Parasites in the Field: The Ever-Innovating CRISPR-Cas12a

^* ,

Version 1 : Received: 21 December 2023 / Approved: 21 December 2023 / Online: 21 December 2023 (11:09:22 CET)

A peer-reviewed article of this Preprint also exists.

Li, X.; Dang, Z.; Tang, W.; Zhang, H.; Shao, J.; Jiang, R.; Zhang, X.; Huang, F. Detection of Parasites in the Field: The Ever-Innovating CRISPR/Cas12a. Biosensors 2024, 14, 145. Li, X.; Dang, Z.; Tang, W.; Zhang, H.; Shao, J.; Jiang, R.; Zhang, X.; Huang, F. Detection of Parasites in the Field: The Ever-Innovating CRISPR/Cas12a. Biosensors 2024, 14, 145.

Abstract

The rapid and accurate detection of parasites is crucial for timely curative intervention in parasitosis and for epidemiological surveillance. To meet the needs of clinical diagnosis, it is imperative to develop a diagnostic tool based on nucleic acid that combines the sensitivity and specificity of established nucleic acid amplifica-tion tests with the speed, cost-effectiveness, and convenience of isothermal amplification methods. A new nu-cleic acid detection method, utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) nuclease, holds promise for point-of-care testing. Specifically, the CRISPR-Cas12a system has demonstrated numerous advantages for detecting parasites, with hopeful outcomes for identify-ing malaria, toxoplasmosis, and other parasitic ailments. This review provides an overview of how CRISPR-Cas12a can be utilized for parasite detection, evaluates its advantages and disadvantages, and sug-gests ways to improve the efficiency and sensitivity of CRISPR-Cas12a-based assays.

Keywords

detection; CRISPR; suboptimal crRNA; light-activated crRNA; tandem repeats; POCT

Subject

Biology and Life Sciences, Parasitology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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