Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of O26 Enteropathogenic E. coli and Its Impli-Cation for Vaccine Development

Thiago Jordão da Silva Lemos
ORCID logo ,
Herbert Guimarães de Sousa Silva
,
José Osvaldo Previato
,
Lucia Mendonça-Previato
ORCID logo ,
Elisangela Oliveira de Freita
,
Angela Silva Barbosa
ORCID logo ,
Marcia Regina Franzolin
ORCID logo ,
Luis Fernando dos Santos
ORCID logo ,
Bruna de Sousa Melo
,
Geovana Ferreira dos Anjos
,
Renata Hiromi Nakagima Gonçalves
,
Marta de Oliveira Domingos
*
Version 1 : Received: 15 December 2023 / Approved: 19 December 2023 / Online: 19 December 2023 (07:55:19 CET)

A peer-reviewed article of this Preprint also exists.

Lemos, T.J.S.; Silva, H.G.S.; Previato, J.O.; Mendonça-Previato, L.; Freitas, E.O.; Barbosa, A.S.; Franzolin, M.R.; Santos, L.F.; Melo, B.S.; Anjos, G.F.; Gonçalves, R.H.N.; Domingos, M.O. O26 Polysaccharides as Key Players in Enteropathogenic E. coli Immune Evasion and Vaccine Development. Int. J. Mol. Sci. 2024, 25, 2878. Lemos, T.J.S.; Silva, H.G.S.; Previato, J.O.; Mendonça-Previato, L.; Freitas, E.O.; Barbosa, A.S.; Franzolin, M.R.; Santos, L.F.; Melo, B.S.; Anjos, G.F.; Gonçalves, R.H.N.; Domingos, M.O. O26 Polysaccharides as Key Players in Enteropathogenic E. coli Immune Evasion and Vaccine Development. Int. J. Mol. Sci. 2024, 25, 2878.

Abstract

O26 enteropathogenic Escherichia coli (EPEC), possesses a unique pseudocapsule composed of polysaccharides identical to the O antigen in their lipopolysaccharide (LPS) membrane. Therefore, the impact of O26 polysaccharides on the immune evasion mechanisms of capsulated O26 EPEC was investigated. Our findings reveal that the concentration of GlcNAc, an immunodominant unit of O26 polysaccharides capable of activating the MBL (Mannose Biding Lecting) pathway of the complement system, was found to be lower in capsulated EPEC O26 compared to naked shiga toxin-producing E. coli (STEC) O26 strain. It was noted that, unlike the naked STEC strain, the capsulated EPEC O26 strain was protected against lysis by the alternative pathway of the com-plement system by inhibiting C3b adhesion to its bacterial surface. Capsulated EPEC O26 strains were also able to avoid phagocytosis by macrophages. However, the immune evasion provided by the capsule was overcome in the presence of anti-O26 polysaccharide antibodies. Additionally, these antibodies were able to recognize O26 EPEC in the presence of biofilm and inhibit their adhesion to human epithelial cells. Overall, the results indicate that the O26 polysaccharides can generate an effective humoral immune response, making them promising antigens for the de-velopment of a vaccine against capsulated O26 E. coli

Keywords

E. coli 1; polysaccharide O26 2; vaccine 3; enteropathogenic E. coli; EPEC; shiga toxin-producing E. coli; STEC; enterohemorrhagic E. coli; EHEC

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.