Lu, Z.; Zhang, L.; Mu, Q.; Liu, J.; Chen, Y.; Wang, H.; Zhang, Y.; Su, R.; Wang, R.; Wang, Z.; Lv, Q.; Liu, Z.; Liu, J.; Li, Y.; Zhao, Y. Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats. Agriculture2024, 14, 487.
Lu, Z.; Zhang, L.; Mu, Q.; Liu, J.; Chen, Y.; Wang, H.; Zhang, Y.; Su, R.; Wang, R.; Wang, Z.; Lv, Q.; Liu, Z.; Liu, J.; Li, Y.; Zhao, Y. Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats. Agriculture 2024, 14, 487.
Lu, Z.; Zhang, L.; Mu, Q.; Liu, J.; Chen, Y.; Wang, H.; Zhang, Y.; Su, R.; Wang, R.; Wang, Z.; Lv, Q.; Liu, Z.; Liu, J.; Li, Y.; Zhao, Y. Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats. Agriculture2024, 14, 487.
Lu, Z.; Zhang, L.; Mu, Q.; Liu, J.; Chen, Y.; Wang, H.; Zhang, Y.; Su, R.; Wang, R.; Wang, Z.; Lv, Q.; Liu, Z.; Liu, J.; Li, Y.; Zhao, Y. Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats. Agriculture 2024, 14, 487.
Abstract
With the continuous update of gene editing technology, great progress has been made in livestock breeding, researchers have produced gene edited pigs, cattle, sheep and other improved livestock. Gene editing technology is a genetic means to knock in, knock out, delete, inhibit, activate or replace specific bases of DNA or RNA sequences at the genome level to achieve accurate modification, and can edit genes at a fixed point without the need for DNA templates. In recent years, although regular clusters of interspaced short palindromic repeats (CRISPR)/Cas9 system mediated gene editing technology has been widely used in animal genetic breeding research, but so far, the system's precise insertion efficiency of foreign genes is not high enough, and there are certain off-target effects, and it is not enough to be used in genome editing of large livestock such as cashmere goats. In this paper, the development status, challenges, application prospects and look into the distance of CRISPR/ Cas9-mediated precisiongene editing technology in livestock breeding were reviewed, in order to provide theoretical reference for livestock gene function analysis, genetic improvement and livestock breeding with local economic characteristics.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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