Version 1
: Received: 13 December 2023 / Approved: 13 December 2023 / Online: 14 December 2023 (08:27:36 CET)
How to cite:
De Jesús Valle, M.J.; Rondon Mujica, A.M.; Zarzuelo Castañeda, A.; Duarte, A.C.; Coutinho, P.; Sánchez Navarro, A. Resveratrol Liposomes for Buccal Formulations: Suitability for Chemoprevention and Treatment of Oral Cancer. Preprints2023, 2023121039. https://doi.org/10.20944/preprints202312.1039.v1
De Jesús Valle, M.J.; Rondon Mujica, A.M.; Zarzuelo Castañeda, A.; Duarte, A.C.; Coutinho, P.; Sánchez Navarro, A. Resveratrol Liposomes for Buccal Formulations: Suitability for Chemoprevention and Treatment of Oral Cancer. Preprints 2023, 2023121039. https://doi.org/10.20944/preprints202312.1039.v1
De Jesús Valle, M.J.; Rondon Mujica, A.M.; Zarzuelo Castañeda, A.; Duarte, A.C.; Coutinho, P.; Sánchez Navarro, A. Resveratrol Liposomes for Buccal Formulations: Suitability for Chemoprevention and Treatment of Oral Cancer. Preprints2023, 2023121039. https://doi.org/10.20944/preprints202312.1039.v1
APA Style
De Jesús Valle, M.J., Rondon Mujica, A.M., Zarzuelo Castañeda, A., Duarte, A.C., Coutinho, P., & Sánchez Navarro, A. (2023). Resveratrol Liposomes for Buccal Formulations: Suitability for Chemoprevention and Treatment of Oral Cancer. Preprints. https://doi.org/10.20944/preprints202312.1039.v1
Chicago/Turabian Style
De Jesús Valle, M.J., Paula Coutinho and Amparo Sánchez Navarro. 2023 "Resveratrol Liposomes for Buccal Formulations: Suitability for Chemoprevention and Treatment of Oral Cancer" Preprints. https://doi.org/10.20944/preprints202312.1039.v1
Abstract
Resveratrol is currently considered an option for chemoprevention and treatment of oral cancer, but the instability and unfavorable pharmacokinetics hinder its use in clinical practice. The present study deals with the preparation of resveratrol liposomes by a method that preserves stability and provides payload high enough for chemoprevention and/or treatment of oral cancer. Different temperatures, RSV concentrations and molar fractions, as well as the presence or absence of vitamin C in the hydration media were assayed. Liposome hydrodynamic diameter, polydispersity and zeta potential were evaluated by dynamic light scattering. In vitro biocompatibility, antioxidant activity and permeability were evaluated using TR146 cells and PermeaPad® membranes. Liposomes were included in hydrogels and films, and their suitability for buccal application was evaluated. According to the results, a temperature of 37ºC was optima to efficiently produce the resveratrol liposomes. Vitamin C was found to influence the liposome size, while resveratrol concentration significantly influenced the entrapment efficiency. High cell biocompatibility and potent intracellular antioxidant activity were observed and the inclusion of vitamin C reinforced the antioxidant effects. High permeation across PermeaPad® membranes and efficient uptake by TR146 cells was proved for resveratrol loaded liposomes. Liposomal hydrogels and films appropriated for buccal application were obtained and the payload of the formulations was high enough to produce mucosa concentrations above resveratrol IC50 values reported for cancer cells.
Keywords
Resveratrol liposomes; resveratrol buccal formulations; solvent-free liposome production; resveratrol for oral cancer; liposomal hydrogels; liposomal films
Subject
Medicine and Pharmacology, Pharmacy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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