Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Exploration of Wendan Pomelo Peel from Four Different Growing Regions by LC-MS and Bioactivity Analysis

Version 1 : Received: 11 December 2023 / Approved: 12 December 2023 / Online: 12 December 2023 (06:54:04 CET)

A peer-reviewed article of this Preprint also exists.

Lu, J.; Wang, W. Exploration of Wendan Pomelo (Citrus maxima (Burm.) Merr. cv. Wentan) Peel from Four Different Growing Regions by Liquid Chromatography–Mass Spectrometry and Bioactivity Analysis. Appl. Sci. 2024, 14, 1200. Lu, J.; Wang, W. Exploration of Wendan Pomelo (Citrus maxima (Burm.) Merr. cv. Wentan) Peel from Four Different Growing Regions by Liquid Chromatography–Mass Spectrometry and Bioactivity Analysis. Appl. Sci. 2024, 14, 1200.

Abstract

Wendan (a type of pomelo), as a popular fruit in China, is less known in other countries. Its peel is widely used in food and traditional medicine. Four different origins of Wendan pomelo were selected, and crude extracts were obtained by the Soxhlet extraction method. The composition of Wendan peel was analyzed by high-performance liquid chromatography-mass spectrometry (HPLC-MS) in positive ion mode. The compounds were identified by searching the Metabolite Link (METLIN), Spectral Database for Organic Compounds (SDBS) and referring to literature reports. A total of 20 compounds were identified among the samples from the four origins, of which 8 compounds were common. The majority of the compounds belonged to the flavonoid and coumarin classes, Meranzin hydrate was identified for the first time in pomelo peel. In vitro antioxidant activity experiments showed that samples from Taizhou, Zhejiang exhibited the highest antioxidant activity in the DPPH, ABTS, and FRAP assays, with values of 0.59 mg/mL, 97.06 μmol TE/g, and 60.62 μmol Fe2+/g, respectively. Samples from Zhangzhou, Fujian showed antioxidant activity second only to the samples from Taizhou, Zhejiang. The sample from Zhangzhou, Fujian Province, showed excellent inhibitory activity in the α-glucosidase inhibition assay (IC50=7.99 mg/mL).

Keywords

Wendan pomelo peel; chemical components; Antioxidant; α-glucosidase

Subject

Biology and Life Sciences, Food Science and Technology

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