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Distinctive Culture Expressions of Enterobacteria Interfering With Isolation of Salmonella spp. During the Application of the Recommended ISO 6579–1:2017
Evangelopoulou, G.; Burriel, A.R.; Solomakos, N. Distinctive Culture Expressions of Enterobacteria Interfering with Isolation of Salmonella spp. during the Application of the Recommended ISO 6579-1:2017. Appl. Sci.2024, 14, 953.
Evangelopoulou, G.; Burriel, A.R.; Solomakos, N. Distinctive Culture Expressions of Enterobacteria Interfering with Isolation of Salmonella spp. during the Application of the Recommended ISO 6579-1:2017. Appl. Sci. 2024, 14, 953.
Evangelopoulou, G.; Burriel, A.R.; Solomakos, N. Distinctive Culture Expressions of Enterobacteria Interfering with Isolation of Salmonella spp. during the Application of the Recommended ISO 6579-1:2017. Appl. Sci.2024, 14, 953.
Evangelopoulou, G.; Burriel, A.R.; Solomakos, N. Distinctive Culture Expressions of Enterobacteria Interfering with Isolation of Salmonella spp. during the Application of the Recommended ISO 6579-1:2017. Appl. Sci. 2024, 14, 953.
Abstract
The objective of the present report is the dissemination of information acquired during the application of ISO 6579-1:2017 for the isolation of Salmonella spp. from swine samples. ISO 6579-1:2017 is the officially recommended by the EU protocol for the isolation of Salmonella spp., aiming in the harmonization of effective successful control of Salmonella infection in food producing animals. Successful control of animal salmonellosis is highly dependent on the sensitivity of the biochemical methods reliably detecting the presence of the pathogen in various stages of food production. Application of ISO 6579-1:2017 resulted in the isolation of twelve Salmonella spp. and eighteen other members of the family of Enterobacteriaceae biochemically and antigenically resembling salmonellae. The findings necessitated in the depth evaluation of the culture media xylose-lysine-desoxycholate agar (XLD), Salmonella-Shigella Agar (SS), Brilliant Green Agar (BGA), Salmonella Chromogenic Agar (SCA), Triple Sugar Iron (TSI) and modified semi-solid Rappaport–Vassiliadis (MSRV) agar. The evaluation showed that these culture selective media differed significantly in their performance regarding the isolation of Salmonella from swine samples. It was concluded that the presence of atypical Salmonella strains negatively affects the prevalence of Salmonella, thus the identification of carrier pigs, eventually affecting the efficiency of control programs. Thus, doubtful results require additional biochemical testing for confirming the accuracy of such universally recommended isolation methods.
Keywords
salmonella; isolation; ISO 6579-1:2017; pre-enrichment; selective media; culture methods; swine
Subject
Public Health and Healthcare, Other
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
